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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pEGFP-C1

Plasmid

#71378

Purpose

For analysis of PH domain localization in mammalian cells

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin homology (PH) domain; aa 247-399

Promoter

CMV

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Arl13b-EGFP

Plasmid

#234844

Purpose

A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Arl13b (ARL13B Human)

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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TOP-GFP.mC

Plasmid

#35491

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xTOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

7xTCF/LEF

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD-Lime

Plasmid

#191255

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein Lime

Depositor

Robert Campbell

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Lime

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_Donor (EMM0021)

Plasmid

#63934

Purpose

3X Flag with P2A-Neo for 3' tagging of endogenous proteins

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Glycine-Serine-3XFlag-P2A-NeoR

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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Human Lipid Droplet and Metabolism Library

Pooled Library

#191535

Purpose

Targets 1,196 genes related to lipid droplet biology and cell metabolism.

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only
GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_mVenus

Plasmid

#113893

Purpose

Mammalian (inducible) expression of cytosolic mVenus

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Venus

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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