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Plasmid#188586PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler N-terminal domain
UseTagsFLAG-MycExpressionInsectMutationdeleted first ATGPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]
Plasmid#188587PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain
UseTagsFLAG-MycExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z3-Nluc
Plasmid#174486PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ3-Nluc
UseTags6x Histidine tagExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D94 (pAF10C6)
Plasmid#185848PurposeExpressing artificial trans-repressor in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>CYC8>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9341 (pgRNA_IX-1_NatMX)
Plasmid#161593PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9343 (pgRNA_XV-1_NatMX)
Plasmid#161595PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9358(VII-1_Markerfree_BackBone)
Plasmid#161599PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9359(VIII-1_Markerfree_BackBone)
Plasmid#161600PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9361(XIII-1_Markerfree_BackBone)
Plasmid#161602PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9363(XVI-1_Markerfree_BackBone)
Plasmid#161604PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB345
Plasmid#185078Purposerfa3D46 truncation fused to GFPDepositorInsertRFA3
UseTagsGFPExpressionYeastMutationRfa3 truncation aa 1-46PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB349
Plasmid#185080Purposerfa2D248 truncation fused to GFPDepositorInsertRFA2
UseTagsGFPExpressionYeastMutationRfa2 truncation aa 1-247PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB202
Plasmid#169113PurposeTESEC expression vector for mEGFPDepositorInsertmEGFP
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4_Rap1
Plasmid#184662PurposeMammalian expression of mouse Rap1DepositorInsertrap1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_340-368-GST-V5His6_AH
Plasmid#148935PurposeInsect Expression of DmGIGYF_340-368DepositorInsertDmGIGYF_340-368 (Gyf Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA.HA.HA.Mm.RAP1
Plasmid#184646PurposeMammalian expression of mouse Rap1DepositorInsertrap1
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin_AD
Plasmid#148531PurposeMammalian Expression of DmRoquinDepositorInsertDmRoquin (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS105
Plasmid#174830PurposeEncodes a unique yeast telomere end at TEL11R by removing the subtelomereDepositorInsert11R Unique
UseTagsGAL4 5XUASExpressionBacterialMutationChanging TEL11R to a unique endPromoterlac promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL30
Plasmid#174064PurposeEncodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.DepositorInsertpGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD 1-147
Plasmid#165819Purpose1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertGal4BD 1-147
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL4AD 768-881
Plasmid#165820Purpose768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertGAL4AD 768-881
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Promoterless TP10
Plasmid#165787PurposeDrosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectorsDepositorInsertPromoterless T10
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only