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NAX_CAGGS_CAS9_VPR_Blasto

Plasmid

#167888

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_iRFP713

Plasmid

#167868

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Hygro

Plasmid

#167867

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EBFP2

Plasmid

#167865

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_iRFP713

Plasmid

#167860

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 21-55 eGFP SV40

Plasmid

#132653

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 41-55 eGFP SV40

Plasmid

#132652

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5 sh1 shRNA

Plasmid

#122227

Purpose

Knock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5 sh1 3m shRNA

Plasmid

#122228

Purpose

Negative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65(100-261)-RTA(75-190)

Plasmid

#99672

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-p65(101-261)-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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IFI16-(192-393)-HinA-E381A

Plasmid

#35058

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E381A mutation

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E272A

Plasmid

#35057

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E272A mutation

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E222A

Plasmid

#35056

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E222A mutation

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-T220A

Plasmid

#35055

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; T220A mutation

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-Y218A

Plasmid

#35054

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; Y218A mutation

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pCMV myc DAF-12(P687S)

Plasmid

#32567

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12(P687S) (daf-12 Nematode)

Tags

myc epitodpe

Expression

Mammalian

Mutation

Proline 687 to Serine

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF2

Plasmid

#200382

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF0

Plasmid

#200380

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF1

Plasmid

#200381

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUCC001

Plasmid

#124451

Purpose

Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HH-BsaI

Expression

Yeast

Mutation

gRNA expression cassette from pUDP002 modified to…

Promoter

TDH3 promoter from S.cerevisiae (already present …

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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