Addgene

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MSCV-Flag-ER-Hoxb8-IRES-GFP

Plasmid

#222292

Purpose

Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

David Sykes, Mark Kamps

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF3-IS5

Plasmid

#65220

Purpose

Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

Depositor

Jeffrey Barrick

Article

Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

Inserts

ndmA
ndmB
ndmC
ndmD
gst9
chlR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed start codon from gtg to atg and Changed s…

Promoter

BBa_J23100

Available since

Jan. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_IKBKG_WT

Plasmid

#82242

Purpose

Gateway Donor vector containing IKBKG , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKBKG (IKBKG Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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