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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
AXBE

Plasmid

#204605

Purpose

Mammalian expression of adenine transversion base editor AXBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

S. pyogenes Cas9 (D10A)

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6

Plasmid

#113887

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Article

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
pHRi_1G4 hTCRβ-SNAP

Plasmid

#187604

Purpose

Lentiviral expression of hTCR beta(1G4), and SNAP for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 hTCR beta, and SNAP tag (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

Mouse Pgk1

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Sema4D-Flag

Plasmid

#190646

Purpose

Expresses Mouse Sema4D with C Terminal Flag Tag

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4d (Sema4d Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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