We narrowed to 15,139 results for: nans

1. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

   Plasmid

   #207160

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLXSN-FLK1 TM

   Plasmid

   #65249

   Purpose

   Expression of dominant negative FLK1 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

   Insert

   FLK1 TM (Kdr Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted AA 786-1346

   Promoter

   LTR

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

   Plasmid

   #130709

   Purpose

   Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
   

   Use

   Tags

   NTOM20 and SNAP-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. L-YARS2-9

   Plasmid

   #166533

   Purpose

   scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. O-MARS-11

   Plasmid

   #166541

   Purpose

   scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCasper-pNos-PCP-eGFP

   Plasmid

   #180274

   Purpose

   to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

   Depositor

   Hernan Garcia

   Article

   Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

   Insert

   PCP-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUASTLOTattB_mCherry-CD8-vhhGFP

   Plasmid

   #163930

   Purpose

   Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pk_CyRPA-Cd4-bio-His

   Plasmid

   #126841

   Purpose

   Expression of recombinant P. knowlesi protein in mammalian cells

   Depositor

   Gavin Wright

   Article

   Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

   Insert

   CyRPA (PKH_052740 Plasmodium knowlesi)
   

   Use

   Tags

   ratCd4(d3+4)-bio-6His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKM-IRES-hrGFP

   Plasmid

   #73405

   Purpose

   Mammalian expression of mouse PKM and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKM (Prkcz Mouse)
   

   Use

   Tags

   3xFLAG and pIRES-hrGFP-2a

   Expression

   Mammalian

   Mutation

   truncated, constitutively active form

   Promoter

   CMV

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-Syn_CapChR1_eYFP

    Plasmid

    #188033

    Purpose

    Expresses CapChR1-eYFP in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    hSyn

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FB027

    Plasmid

    #119712

    Purpose

    Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. L-NARS-2

    Plasmid

    #166532

    Purpose

    scFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. O-AARS-51

    Plasmid

    #166539

    Purpose

    scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-455 of 968

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L-TARS-11

    Plasmid

    #166536

    Purpose

    scFv of a human scaffold targeting Threonyl-tRNA synthetase. Antigen coverage aa 320-723 of 723

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L-WARS-26

    Plasmid

    #166528

    Purpose

    scFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DCT.VV.Orf166

    Plasmid

    #12854

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf166
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pJN601

    Plasmid

    #59790

    Purpose

    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128342

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna7 (Chrna7 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSP1D1T2

    Plasmid

    #97328

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T2
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of two T residues after T25

    Promoter

    T7

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128346

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Rbfox3 (NeuN) (Rbfox3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge