We narrowed to 5,840 results for: pCas

1. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav2.2 HA EIVK (E1658K) pCAGGS

   Plasmid

   #206096

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   E1658K

   Promoter

   CMV/B-actin

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-MP-84-spCas9-U6-gRNA-tlock

   Plasmid

   #206936

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   MP-84

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-FIP200 (1-640)-GST

   Plasmid

   #203544

   Purpose

   FIP200 NTD-MBP mammalian expression

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GST-ATG13 (6-196)

   Plasmid

   #203559

   Purpose

   Mammalian expression of GST tagged ATG13 (6-196)

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-SMS1-TEV-HAT

   Plasmid

   #202557

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS1 (SGMS1 Human)
   

   Tags

   Histidine-Affinity-tag (HAT) and TEV cleavable si…

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 USP8, residues 1-147

   Plasmid

   #180636

   Purpose

   Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   USP8 (USP8 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-147

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CALPAIN-7 residues 1-165

   Plasmid

   #180611

   Purpose

   Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-165

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 KATNA1 MIT residues 1-79

   Plasmid

   #180614

   Purpose

   Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-79

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 KATNAL1 MIT residues 1-87

    Plasmid

    #180618

    Purpose

    Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNAL1 (KATNAL1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-87

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-KATNA1 R14A

    Plasmid

    #160052

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    R14A mutation

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-OSF-KATNA1 V55D

    Plasmid

    #160053

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    V55D mutation

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-msfGFP-ATG13 (1-197)

    Plasmid

    #203560

    Purpose

    Mammalian expression of GFP tagged ATG13 (1-197)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-AaCas12b-T2A-EGFP

    Plasmid

    #188271

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    AaCas12b
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-PlmCasX-T2A-EGFP

    Plasmid

    #188273

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-TcCas12b-T2A-EGFP

    Plasmid

    #188277

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

    Insert

    TcCas12b
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGG-GST-TEV-NAP1 (WT)

    Plasmid

    #198034

    Purpose

    Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    GST-TEV

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

    Plasmid

    #148897

    Purpose

    Mammalian Expression of HsHelz-sgRNA

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz-sgRNA (HELZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

    Plasmid

    #148848

    Purpose

    Mammalian Expression of HsAGO2-sgRNA1

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO2-sgRNA1 (AGO2 S.pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

    Plasmid

    #148849

    Purpose

    Mammalian Expression of HsTNRC6A-sgRNA1

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6A-sgRNA1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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