1. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-spCas9-SB

   Plasmid

   #169919

   Purpose

   Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-SB100X
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-P2A_BAF

   Plasmid

   #101773

   Purpose

   Expresses BAF in human cells (with EGFP produced from the same transcript as expression control)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFP-P2A_BAF_G47E

   Plasmid

   #101774

   Purpose

   Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP-P2A

   Expression

   Mammalian

   Mutation

   G47E mutation

   Promoter

   EF1a

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EGFP-P2A_BAF_L58R

   Plasmid

   #101775

   Purpose

   Expresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP-P2A

   Expression

   Mammalian

   Mutation

   L58R mutation

   Promoter

   EF1a

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-AsCpf1-Blast

   Plasmid

   #84750

   Purpose

   Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   AsCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208041

    Purpose

    Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    TurboID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4134186

    Plasmid

    #152962

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;TapTag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Lenti-pHSE::Cre-mRuby3

    Plasmid

    #164136

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    HSE promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSE synthetic promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-pNFkB::Cre-mRuby3

    Plasmid

    #164137

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    NFkB promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NFkB synthetic promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208040

    Purpose

    Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, TurboID

    Expression

    Mutation

    Promoter

    EFS

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Expression

    Mutation

    Promoter

    EFS

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208038

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Expression

    Mutation

    Promoter

    EFS

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge