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pBABE HA Chk2 KD, dominant negative chk2
Plasmid#41902DepositorInsertChk2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationD347APromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-HK2
Plasmid#23854DepositorInsertHK2 (HK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
MmSphk2
Plasmid#118598PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDHK29
Plasmid#80783PurposeCloning vector compatible with ColE1 plasmids with high copy numberDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK2
Plasmid#118092PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 2 (SPHK2 Human)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHK2_HUMAN_D0
Plasmid#79715PurposeThis plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCHK2 (CHEK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 WT
Plasmid#41901DepositorInsertCHK2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorInsertSPHK2 (SPHK2 Human)
UseTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgHK2_2
Plasmid#163461Purposelentiviral vector expressing Cas9 and an sgRNA targeting HK2DepositorInsertsgRNA 2 targeting HK2 (HK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-HK2_sgRNA1
Plasmid#201596PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertHK2 (HK2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SPHK2
Plasmid#23714DepositorInsertSPHK2 (SPHK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-HK2_sgRNA2
Plasmid#201597PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertHK2 (HK2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPHK2 gRNA (BRDN0001148465)
Plasmid#77093Purpose3rd generation lentiviral gRNA plasmid targeting human SPHK2DepositorInsertSPHK2 (SPHK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-HK2-3xFLAG
Plasmid#163451Purposelentiviral overexpression vector for human HK2DepositorInsertHK2 (HK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hSphK2-EGFP
Plasmid#84371PurposeExpression of exogenous human SphK2 in mammalian cellsDepositorInsertSPHK2 (SPHK2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
HK2 gRNA (BRDN0001162212)
Plasmid#76310Purpose3rd generation lentiviral gRNA plasmid targeting human HK2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-HA-Chk2-D311V
Plasmid#41921DepositorInsertChk2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationD311VPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
SPHK2 gRNA (BRDN0001146242)
Plasmid#77095Purpose3rd generation lentiviral gRNA plasmid targeting human SPHK2DepositorInsertSPHK2 (SPHK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hchk2 (kin-)-PUNI10
Plasmid#41908DepositorInsertCHK2 (CHEK2 Human)
UseTagsExpressionBacterialMutationkinase dead (D347A)PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only
