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Addgene
Showing: 21 - 40 of 598 results

  1. HCA2-Tango

    Plasmid
    #66396
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    66396-plasmid-map-sequence-id-372748

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  3. pBRCA2.1.0-gDNA

    Plasmid
    #132448
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    BRCA2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    132448-plasmid-map-sequence-id-266805

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  5. HCA2-DuET

    Plasmid
    #213308
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213308-plasmid-map-sequence-id-430846

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  6. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (AAV5)

    Viral Prep
    #111568-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (#111568). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-mCherry-hPMCA2w/b plasmid DNA. GfaABCD1-driven, expression of mCherry fused plasma membrane calcium pump hPMCA2w/b. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)
    Promoter
    Tags
    mCherry
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 598 results