Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 281 - 300 of 4879 results

  5. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Article
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    109399-plasmid-map-sequence-id-211963

    Enlarge

  9. pJK436

    Plasmid
    #72477
    Purpose
    Produces Thermoplasma acidophilum citrate synthase
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72477-plasmid-map-sequence-id-134619

    Enlarge

  10. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

    Enlarge

  11. pJK383

    Plasmid
    #72474
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-17 to phenylalanine, tryptopha…
    Promoter
    recA
    Availability
    Academic Institutions and Nonprofits only
    72474-plasmid-map-sequence-id-140430

    Enlarge

  13. pJK511

    Plasmid
    #71591
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)
    Depositor
    T. J. Kappock
    Article
    Murphy et al Acta Crystallogr F Struct Biol Commun. 2
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes histidine-222 to glutamine
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    71591-plasmid-map-sequence-id-130911

    Enlarge

Showing: 281 - 300 of 4879 results