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Showing: 1 - 20 of 27 results

  2. RHPMP190 (pLifeAct tagRFP-T)

    Plasmid
    #88842
    Purpose
    Localization by Fluorescence microscopy of lifeAct fused to tagRFP-T
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    tagRFP-T
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    88842-plasmid-map-sequence-id-177512

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  6. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  10. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

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  13. pcDNA3-Lyn-μDpTT

    Plasmid
    #182321
    Purpose
    Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lyn-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182321-plasmid-map-sequence-id-362887

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  14. pDronpa-N1-Tau-μDpTT

    Plasmid
    #182323
    Purpose
    Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Tau-μDronpa-TagRFP-T
    Use
    Tags
    MAP tau
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182323-plasmid-map-sequence-id-363424

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  15. pcDNA3-DAKAP-μDpTT

    Plasmid
    #182324
    Purpose
    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 30 amino acids from DAKAP1
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182324-plasmid-map-sequence-id-362788

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  16. pcDNA3-CYP450-μDpTT

    Plasmid
    #182325
    Purpose
    Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 27 amino acids from CYP450
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182325-plasmid-map-sequence-id-362816

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  17. pcDNA3-Lifeact-μDpTT

    Plasmid
    #182322
    Purpose
    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lifeact-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 17 amino acids from budding yeast Abp1…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182322-plasmid-map-sequence-id-362772

    Enlarge

  18. pcDNA3-RIIα-μDpTT

    Plasmid
    #182327
    Purpose
    PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
    Use
    Tags
    Full-length human PKA type 2alpha regulatory subu…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182327-plasmid-map-sequence-id-363422

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  19. pcDNA3-H2B-μDpTT

    Plasmid
    #182328
    Purpose
    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    Use
    Tags
    Full-length human histone H2B
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182328-plasmid-map-sequence-id-363396

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  20. pcDNA3-β2AR-μDpTT

    Plasmid
    #182326
    Purpose
    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    Use
    Tags
    Full-length human beta2 adrenergic receptor
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182326-plasmid-map-sequence-id-366176

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Showing: 1 - 20 of 27 results