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Showing: 1 - 20 of 174 results

  1. pBA2675

    Plasmid
    #189997
    Purpose
    Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)
    Depositor
    Bungo Akiyoshi
    Article
    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    NLS
    Expression
    Mutation
    N-terminal 600 bp of FBP75
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    189997-plasmid-map-sequence-id-371586

    Enlarge

  2. pBA2705

    Plasmid
    #189998
    Purpose
    Inducible expression of FBP75(1–200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)
    Depositor
    Bungo Akiyoshi
    Article
    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    Expression
    Mutation
    N-terminal 600 bp of FBP75
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    189998-plasmid-map-sequence-id-371593

    Enlarge

  5. PP-ASL

    Plasmid
    #40749
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Trypanosoma Brucei Adenylosuccinate Lyase
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  6. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Depositor
    Kenneth Christensen
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Availability
    Academic Institutions and Nonprofits only
    213776-plasmid-map-sequence-id-423197

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  7. pUB39

    Plasmid
    #26300
    Depositor
    George Cross
    Insert
    VSG117 (Lister 427-4) cDNA
    Use
    Trypanosome inducible expression vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26300-plasmid-map-sequence-id-12384

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  10. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    68709-plasmid-map-sequence-id-125636

    Enlarge

  11. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Gloria Rudenko
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressi…
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    59733-plasmid-map-sequence-id-93744

    Enlarge

  12. 6NEY

    Plasmid
    #127295
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    127295-plasmid-map-sequence-id-247860

    Enlarge

  13. TBSAHA

    Plasmid
    #42436
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TBSAHA
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  14. pRPa-c6MYCn

    Plasmid
    #69242
    Purpose
    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Availability
    Academic Institutions and Nonprofits only
    69242-plasmid-map-sequence-id-123680

    Enlarge

  15. pRP-c6MYCn

    Plasmid
    #69243
    Purpose
    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Availability
    Academic Institutions and Nonprofits only
    69243-plasmid-map-sequence-id-123691

    Enlarge

  19. 6NIM

    Plasmid
    #127294
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD18
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    127294-plasmid-map-sequence-id-247944

    Enlarge

  20. PP-LeuAP

    Plasmid
    #40750
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    M17 aminopeptidase
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 174 results