We narrowed to 34 results for: wandless

1. pBMN FKBP(DD)-YFP

   Plasmid

   #31763

   Depositor

   Thomas Wandless

   Article

   Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

   Insert

   FKBP(DD)-YFP-HA (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   F36V, L106P on FKBP12

   Promoter

   LTR

   Available since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b His6-FKBPF36V

   Plasmid

   #73180

   Purpose

   Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

   Depositor

   Thomas Wandless

   Article

   Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

   Insert

   FKBP(F36V) (FKBP1A Human)
   

   Use

   Tags

   His 6x

   Expression

   Bacterial

   Mutation

   F36V

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pBMN YFP-DHFR(DD)

   Plasmid

   #29326

   Depositor

   Thomas Wandless

   Article

   Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

   Insert

   YFP-DHFR(DD)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBMN YFP-FKBP(DD)

   Plasmid

   #31766

   Depositor

   Thomas Wandless

   Article

   Chu et al Bioorg Med Chem Lett. 2008 Nov 15;18(22)

   Insert

   HA-YFP-FKBP(DD) (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   E31G, F36V, R71G and K105E on FKBP12

   Promoter

   LTR

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AgDD

   Plasmid

   #78289

   Purpose

   create protein aggregates in living cells

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Use

   Tags

   MLALKLAGLDI

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBMN DHFR(DD)-YFP

   Plasmid

   #29325

   Depositor

   Thomas Wandless

   Article

   Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

   Insert

   DHFR(DD)-YFP
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

   Promoter

   Available since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR

   Plasmid

   #24585

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP

   Plasmid

   #170157

   Purpose

   Expression for LIBRON constructs

   Depositor

   Thomas Wandless

   Article

   Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

   Insert

   FKBP DD (L106P)-UbK0G75S
   

   Use

   Retroviral

   Tags

   sfGFP

   Expression

   Mutation

   change all lysin residues of ubiquitin to Arg, G7…

   Promoter

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBMN YFP-LID

   Plasmid

   #31767

   Depositor

   Thomas Wandless

   Article

   Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

   Insert

   YFP-LID (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

   Promoter

   LTR

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBMN HAYFP-LOV24

    Plasmid

    #49570

    Purpose

    regulate protein stability with light

    Depositor

    Thomas Wandless

    Article

    Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

    Insert

    YFP-LOV24
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    delete the last 3 aa from AsLOV2, with I532A muta…

    Promoter

    LTR

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP

    Plasmid

    #172001

    Purpose

    Expression for LIBRON constructs

    Depositor

    Thomas Wandless

    Article

    Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

    Insert

    FKBP DD (L106P)-UbK0G75C
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mutation

    change all lysin residues of ubiquitin to Arg, G7…

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-FDD

    Plasmid

    #80629

    Purpose

    Fluorescent destablizing domain for regulating protein stability and fluorescence

    Depositor

    Thomas Wandless

    Article

    Navarro et al ACS Chem Biol. 2016 Jun 6.

    Insert

    UnaG
    

    Use

    Retroviral

    Tags

    HA-mcherry-linker

    Expression

    Mutation

    mutations A36V, R136G

    Promoter

    LTR

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NLS-AgDD

    Plasmid

    #80625

    Purpose

    create nuclear protein aggregates in mammalian cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NLS-NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MPPKKKRKVLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR

    Plasmid

    #24378

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::p150yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET15b His6-ecDHFR (WT)

    Plasmid

    #73188

    Purpose

    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

    Depositor

    Thomas Wandless

    Insert

    folA dihydrofolate reductase (folA E. coli)
    

    Use

    Tags

    His 6X

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

    Plasmid

    #24584

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

    Plasmid

    #24549

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pScDD2_ERdd

    Plasmid

    #109047

    Purpose

    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

    Depositor

    Thomas Wandless

    Insert

    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    

    Use

    Tags

    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

    Expression

    Yeast

    Mutation

    3 mutations : E380G, R434G, N532S

    Promoter

    SOD1

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PBMN YFP-ER50

    Plasmid

    #37261

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al J Am Chem Soc. 2012 Mar 7;134(9):3942-5.

    Insert

    ER50 (ESR1 Human)
    

    Use

    Retroviral

    Tags

    HA-YFP

    Expression

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Promoter

    LTR

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pvha6-AgDD

    Plasmid

    #80626

    Purpose

    create protein aggregates in C. elegans

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge