158 results
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Plasmid Kit#1000000098PurposeYeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9DepositorAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only
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Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
The Expanded EasyClone-MarkerFree toolkit
Plasmid Kit#1000000190PurposeThe Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.DepositorAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Multiplex CRISPR/Cas9 Assembly System Kit
Plasmid Kit#1000000055PurposeGolden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory PackDepositorAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Phosphoinositide Biosensors Kit
Plasmid Kit#1000000261PurposeUse the phosphoinositide biosensors kit to detect the whole family of phosphoinositides in fixed cells and tissues.DepositorAvailable sinceJuly 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH
Plasmid Kit#1000000163PurposeA collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCRDepositorAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
The Mammalian Toolkit
Plasmid Kit#1000000180PurposeA ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.DepositorAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ONE-GO biosensors
Plasmid Kit#1000000224PurposeThis kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).DepositorAvailable sinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Sybody Generation Toolbox
Plasmid Kit#1000000160PurposeThe Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.DepositorAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Parts Kit
Plasmid Kit#1000000059PurposeCIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coliDepositorAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MoClo Pichia toolkit
Plasmid Kit#1000000108PurposeA collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).DepositorAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pro-Code Vector Kit
Plasmid Kit#1000000177PurposeThe Pro-Code Vector Kit is a set of 120 lentiviral vector plasmids, each with a unique protein barcode (Pro-Code) and gRNA cloning site.These plasmids can be used for CRISPR screens.DepositorAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Chromoprotein kit 1.0
Plasmid Kit#1000000174PurposeA synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.DepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GreenGate 2.0 Toolkit
Plasmid Kit#1000000241PurposeThe GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.DepositorAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain Constructs for Automated Bacterial Expression
Plasmid Kit#1000000094Purpose68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expressionDepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TALE Toolbox
Plasmid Kit#1000000019PurposeProvides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.DepositorAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Marionette Sensor Collection
Plasmid Kit#1000000137PurposeThe Marionette Sensor Collection enables the modular control of up to 12 genes, by placing each one under the control of a small inducible promoter.DepositorAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
biGBac Kit
Plasmid Kit#1000000088PurposeGeneration of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.DepositorAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mobius Assembly Vector Toolkit
Plasmid Kit#1000000134PurposeMobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate partsDepositorAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only