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Showing: 101 - 150 of 152 results

  1. Rinehart & Söll Phosphoprotein Synthesis Kit

    Plasmid Kit
    #1000000020
    Purpose
    Allows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate Sep–tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).
    Depositor
    Jesse Rinehart, Dieter Söll
    Available since
    March 1, 2012
    Availability
    Academic Institutions and Nonprofits only

  2. Vaccinia Virus ORF Library

    Plasmid Kit
    #1000000009
    Purpose
    Vaccinia virus (VACV) expression library containing each of the predicted open reading frames based on an unpublished sequence of vaccinia virus strain WR
    Depositor
    Jon Yewdell, Jack Bennink
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  3. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.
    Depositor
    Keith Joung
    Available since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. Myristoylated Kinase Library

    Plasmid Kit
    #1000000012
    Purpose
    190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.
    Depositor
    William Hahn
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.
    Depositor
    Takashi Yamamoto
    Available since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Zinc Finger Consortium Expression Vector and Modular Assembly Kit

    Plasmid Kit
    #1000000005
    Purpose
    Can be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.
    Depositor
    Keith Joung
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    This three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.
    Depositor
    Lisbeth Olsson
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  8. pGCS vector kit

    Plasmid Kit
    #1000000107
    Purpose
    A series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.
    Depositor
    Hai-Ning Du
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. Target Accelerator Genetic Pathway Reference Set

    Plasmid Kit
    #1000000105
    Purpose
    Collection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processes
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. MultiSite Gateway plasmid toolKit

    Plasmid Kit
    #1000000050
    Purpose
    Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules
    Depositor
    Tomas Santalucia
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid Kit
    #1000000010
    Purpose
    Contains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.
    Depositor
    Keith Joung
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation
    Depositor
    Veit Hornung
    Available since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    The modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. Hamdoun lab FP fusion plasmids

    Plasmid Kit
    #1000000033
    Purpose
    Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)
    Depositor
    Amro Hamdoun
    Available since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Start-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.
    Depositor
    John Heap
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. Target Accelerator Pan-Cancer Mutant Collection

    Plasmid Kit
    #1000000103
    Purpose
    Panel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning system
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. BIOFAB

    Plasmid Kit
    #1000000037
    Purpose
    Sets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interest
    Depositor
    Drew Endy
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems
    Depositor
    Dominic Esposito
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  20. Zinc Finger Consortium OPEN Accessory Reagent Set

    Plasmid Kit
    #1000000013
    Purpose
    Contains plasmids and strains used to engineer Zinc Finger Nucleases (ZFNs) via the Oligomerized Pool ENgineering (OPEN) method.
    Depositor
    Keith Joung
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  21. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques
    Depositor
    Thomas Lahaye
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  22. AspFlex Kit

    Plasmid Kit
    #1000000217
    Purpose
    This collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.
    Depositor
    Veronica Godoy-Carter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  23. cpFRET

    Plasmid Kit
    #1000000021
    Purpose
    circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
    Depositor
    Olivier Pertz
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. CRISPRecise Kit

    Plasmid Kit
    #1000000235
    Purpose
    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.
    Depositor
    Ervin Welker
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  25. STAR TALE Assembly Kit

    Plasmid Kit
    #1000000117
    Purpose
    These plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.
    Depositor
    Steve Pollard
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  26. Plasmid-ID Kit

    Plasmid Kit
    #1000000168
    Purpose
    The Plasmid-ID Kit allows for the high-throughput assessment of competitive and effective rhizobial strains.
    Depositor
    Philip Poole
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only

  27. OVC Core Collection

    Plasmid Kit
    #1000000164
    Purpose
    Three plasmids with the empty cloning cassette in configurations ABC, BAC or ACB, and 21 plasmids containing one of nine light-sensing domains (LSDs) or LSD binding partners.
    Depositor
    Harald Janovjak
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  28. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate parts
    Depositor
    Naomi Nakayama
    Available since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  29. Barcoded self-reporting transposon calling card collection

    Plasmid Kit
    #1000000213
    Purpose
    'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.
    Depositor
    Rob Mitra
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only

  30. Musunuru-Cowan TALEN Kit

    Plasmid Kit
    #1000000034
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.
    Depositor
    Kiran Musunuru
    Available since
    July 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  32. dRMCE Plasmids

    Plasmid Kit
    #1000000015
    Purpose
    Takes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous loci
    Depositor
    Rolf Zeller
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  33. FREQ-Seq Kit

    Plasmid Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Christopher Marx
    Available since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. Ekker Lab TALEN kit

    Plasmid Kit
    #1000000038
    Purpose
    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis
    Depositor
    Stephen Ekker
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  36. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  37. Winslow Lab Retroviral Barcoding Library

    Plasmid Kit
    #1000000097
    Purpose
    Barcoding library of retroviral plasmids with unique 6 nucleotide barcodes, for in vivo phenotypic screening of small-molecule libraries.
    Depositor
    Monte Winslow
    Available since
    Dec. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  40. Integrated Genomics Plasmids

    Plasmid Kit
    #1000000004
    Purpose
    Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable
    Depositor
    Guy Caldwell
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  41. The Expanded CRISPEY Kit

    Plasmid Kit
    #1000000257
    Purpose
    Use the Expanded CRISPEY Kit for CRISPR-based gene editing of S. cerevisiae.
    Depositor
    Jeffrey Lewis
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only

  42. TUNEYALI Kit

    Plasmid Kit
    #1000000255
    Purpose
    Use the TUNEYALI kit for precise regulation of transcription factor expression in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only

  43. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.
    Depositor
    Mikhail Shapiro
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  44. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.
    Depositor
    Stephan Gruber
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  45. MultiGreen

    Plasmid Kit
    #1000000250
    Purpose
    The MultiGreen kit enables users to assemble GreenGate-compatible multigene plant transformation vectors.
    Depositor
    Wayne Parrott
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  46. T2A Split/Link TurboID Kit

    Plasmid Kit
    #1000000249
    Purpose
    The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  47. Ubiquiton Human Kit

    Plasmid Kit
    #1000000244
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  48. Ubiquiton Yeast Kit

    Plasmid Kit
    #1000000243
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  49. Ubiquiton In Vitro Kit

    Plasmid Kit
    #1000000242
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  50. Easy-MISE toolkit

    Plasmid Kit
    #1000000230
    Purpose
    The Easy-Modular Integrative fuSion-ready Expression (Easy-MISE) toolkit is a complete vector set leading to fast engineering procedures for the improvement of heterologous production in S. cerevisiae
    Depositor
    Paola Branduardi
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 150 of 152 results