27,466 results
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Plasmid#66222PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAGTR1 (AGTR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_ST-SBP-EGFP
Plasmid#65264Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p189
Plasmid#65207PurposeMutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
ExpressionBacterial and MammalianMutationPremature stop codon added to pGEM5Z(+)-EGFP (TGG…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP rbFOX2
Plasmid#63086PurposeMammalian expression of EGFP tagged FOX2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBFOX2 (RBFOX2 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC-H1-gRNA
Plasmid#61089PurposeCRISPR/Cas9 system gRNA cloningDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH1 promoter; gRNA sequences
UseCRISPR; /cas9 grna cloning vectorExpressionMammalianPromoterH1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-EB3-7
Plasmid#55037PurposeLocalization: MT End Binding Protein, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB3 (MAPRE3 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Smoothened1-N-18
Plasmid#55134PurposeLocalization: Cilium, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSmoothened1
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C3-Sec8
Plasmid#53758PurposepEGFP-C3 plasmid that expresses Sec8DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec8 (EXOC4 Human)
TagsGFPExpressionMammalianAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP_Rab35 Q67L active
Plasmid#47425DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab35 Q67L (RAB35 Human)
TagsGFPExpressionMammalianMutationQ67L activePromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
P1-EYFP-pA (Construct 5)
Plasmid#55197PurposeSynthetic promoter responsive to gRNA1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEYFP
UseSynthetic BiologyExpressionMammalianPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEmerald-Ezrin-N-14
Plasmid#54090PurposeLocalization: Focal Adhesions/Membrane, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEzrin (EZR Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby-Lifeact-7
Plasmid#54560PurposeLocalization: Actin, Excitation: 558, Emission: 605DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-mRuby
ExpressionMammalianAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
delta DE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52381PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianMutationdeleted -3194 - -1768 upstream from OCT4 ATGPromoterOCT4Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
TagsHAExpressionMammalianPromoterEF1-alphaAvailable sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSV-PKI-v2
Plasmid#45066PurposeExpression of PKI (PKA inhibitor)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMP-dependent protein kinase inhibitor alpha
ExpressionMammalianPromoterRSVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA Depdc5 pRK5
Plasmid#46327DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDepdc5 (DEPDC5 Human)
TagsHAExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-HDAC4 3SA
Plasmid#45637DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHDAC4 (HDAC4 Human)
TagsEGFPExpressionMammalianMutation3SAPromoterCMVAvailable sinceJune 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF13E
Plasmid#42942DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEN- Human L1 element (L1RE1 Human)
TagsEGFP (opposite strand)ExpressionMammalianMutationAgeI-BclI fragment swapped with nt 1927-3708 from…PromoterCMV MIE, L1 5'UTRAvailable sinceApril 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF12J
Plasmid#42943DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEN+ Human L1 element (L1RE1 Human)
TagsEGFP (opposite strand)ExpressionMammalianMutationAgeI-BclI fragment swapped with nt 1927-3708 from…PromoterCMV MIE, L1 5'UTRAvailable sinceApril 25, 2013AvailabilityAcademic Institutions and Nonprofits only