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27,466 results

Showing: 11321 - 11340 of 27466 results

  2. pDEST40-2XHA-Src-K298M

    Plasmid
    #140314
    Purpose
    Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies
    Depositor
    Mark Moasser
    Publication
    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Src mutant (SRC Human)
    Tags
    2X HA
    Expression
    Mammalian
    Mutation
    K298M
    Promoter
    CMV
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140314-plasmid-map-sequence-id-291897

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  4. pcDNA3-sACE2v2-sfGFP

    Plasmid
    #145173
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145173-plasmid-map-sequence-id-292589

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  5. iRFP-FRB-ER

    Plasmid
    #139317
    Purpose
    endoplasmic reticulum-resident FRB for chemically induced dimerization
    Depositor
    Gerry Hammond
    Publication
    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)
    Expression
    Mammalian
    Available since
    April 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139317-plasmid-map-sequence-id-287405

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  6. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid
    #139992
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139992-plasmid-map-sequence-id-275524

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  7. Golgi-targeted optoPLD

    Plasmid
    #140058
    Purpose
    Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression
    Depositor
    Jeremy Baskin
    Publication
    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phospholipase D
    Tags
    CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…
    Expression
    Mammalian
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140058-plasmid-map-sequence-id-275745

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  9. ABE8.17-d

    Plasmid
    #136299
    Purpose
    expresses ABE8.17-d in mammalian cells
    Depositor
    Nicole Gaudelli
    Publication
    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE8.20-d
    Tags
    C-terminal BPNLS
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    136299-plasmid-map-sequence-id-269979

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  12. YE2-BE4max

    Plasmid
    #138156
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138156-plasmid-map-sequence-id-272343

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  13. pBTK501

    Plasmid
    #110602
    Purpose
    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)
    Depositor
    Jeffrey Barrick
    Publication
    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP
    Use
    Synthetic Biology
    Available since
    Feb. 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    110602-plasmid-map-sequence-id-215545

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  15. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid
    #127390
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)
    Depositor
    Ryohei Yasuda
    Publication
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Alanine
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127390-plasmid-map-sequence-id-250429

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  16. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid
    #133339
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA_ctrA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133339-plasmid-map-sequence-id-269541

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  18. hCMV-IE1:GrRenilla

    Plasmid
    #118067
    Purpose
    Transcriptional unit for constitutive expression of GrRenilla in mammalian cells
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV:GreenRenilla:bGHF
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118067-plasmid-map-sequence-id-240499

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Showing: 11321 - 11340 of 27466 results