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27,466 results

Showing: 11941 - 11960 of 27466 results

  1. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid
    #171800
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Publication
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeonGreen-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171800-plasmid-map-sequence-id-341952

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  2. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid
    #173871
    Purpose
    Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    Tags
    C-terminal CAAX-box and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173871-plasmid-map-sequence-id-341721

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  3. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid
    #164903
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
    Depositor
    Takeshi Imai
    Publication
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Expression
    Mammalian
    Mutation
    Y40C mutation in the Rac1 domain whcih make the s…
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164903-plasmid-map-sequence-id-340567

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  4. pUC57-Amp_CRISPIE donor B5

    Plasmid
    #172846
    Purpose
    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172846-plasmid-map-sequence-id-340979

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  5. pUC57-Amp_CRISPIE donor B1

    Plasmid
    #172842
    Purpose
    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172842-plasmid-map-sequence-id-340939

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  7. TfR-moxGFP-DHFR

    Plasmid
    #173012
    Purpose
    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-moxGFP-DHFR
    Tags
    DHFR and moxGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    173012-plasmid-map-sequence-id-339204

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  13. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Publication
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Promoter
    RPBSA and TRE promoter
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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  14. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    170818-plasmid-map-sequence-id-334731

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  16. pCG-TPMV Hd32-IL-13

    Plasmid
    #163869
    Purpose
    Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells
    Depositor
    Jakob Reiser
    Publication
    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPMV H glycoprotein/IL-13
    Tags
    Human IL-13
    Expression
    Mammalian
    Mutation
    Amino acids 2 to 33 at N terminus deleted
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163869-plasmid-map-sequence-id-325508

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Showing: 11941 - 11960 of 27466 results