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27,006 results

Showing: 5721 - 5740 of 27006 results

  4. pTetON-DeAct-SpvB

    Plasmid
    #89455
    Purpose
    Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    89455-plasmid-map-sequence-id-176825

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  5. hNedd4-2

    Plasmid
    #83433
    Purpose
    full length Nedd4L with His Xpress tag
    Depositor
    Christie Thomas
    Publication
    Itani et al Am J Physiol Renal Physiol. 2005 Aug;289
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Nedd4L with C2 domain (NEDD4L Human)
    Tags
    His Tag (6x) and Xpress Epitope Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83433-plasmid-map-sequence-id-158994

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  10. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid
    #84245
    Purpose
    Expresses direct fusion KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    KRAB-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    84245-plasmid-map-sequence-id-160888

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  11. DT0

    Plasmid
    #80418
    Purpose
    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Publication
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80418-plasmid-map-sequence-id-151407

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  17. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-targeting sgRNA human (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71830-plasmid-map-sequence-id-131025

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  18. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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Showing: 5721 - 5740 of 27006 results