Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 10141 - 10160 of 27462 results

  4. pNR4A1-donor

    Plasmid
    #112295
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    Use
    CRISPR
    Mutation
    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112295-plasmid-map-sequence-id-228416

    Enlarge

  12. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    John Ngo
    Publication
    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-NS3-NLS/VPR
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112244-plasmid-map-sequence-id-216918

    Enlarge

  13. pEYFP-N1-eKR2

    Plasmid
    #115337
    Purpose
    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse
    Depositor
    Peter Hegemann
    Publication
    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eKR2
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    115337-plasmid-map-sequence-id-222075

    Enlarge

  14. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

    Plasmid
    #111744
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q30 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111744-plasmid-map-sequence-id-220482

    Enlarge

  16. BK beta4 EGFP

    Plasmid
    #113560
    Purpose
    human BK beta4 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kcnmb4 (KCNMB4 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113560-plasmid-map-sequence-id-218925

    Enlarge

  17. Gata3-T2A-H2B-eGFP donor

    Plasmid
    #113119
    Purpose
    Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gata3 donor region including homology arms and T2A-H2B-eGFP
    Use
    Synthetic Biology
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113119-plasmid-map-sequence-id-217796

    Enlarge

  19. Ctcf-Halo-mAID donor

    Plasmid
    #113103
    Purpose
    Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)
    Use
    Synthetic Biology
    Available since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113103-plasmid-map-sequence-id-217898

    Enlarge

  20. pSBbi-FoxO1_1R_13A_3D

    Plasmid
    #106279
    Purpose
    Fluorescent fusion protein for FoxO1
    Depositor
    Laura Heiser
    Publication
    Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Foxo1a (Foxo1 Mouse)
    NLS-mCherry-NLS-P2A-Puromycin
    Tags
    Clover fluorescent protein and NLS
    Expression
    Mammalian
    Mutation
    Deleted amino acids 401-636, T24A, S209A, H212R, …
    Promoter
    EF1a/RPBSA
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    106279-plasmid-map-sequence-id-205981

    Enlarge

Showing: 10141 - 10160 of 27462 results