Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 11881 - 11900 of 27462 results

  6. POLArISact

    Plasmid
    #164971
    Purpose
    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells
    Depositor
    Sumio Terada
    Publication
    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLArISact
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    164971-plasmid-map-sequence-id-326985

    Enlarge

  10. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160140-plasmid-map-sequence-id-317331

    Enlarge

  13. Nup133_mEGFP(-8a)

    Plasmid
    #163417
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nup133 (NUP133 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163417-plasmid-map-sequence-id-321553

    Enlarge

  16. pCMV_eA3A-NG-nCas9

    Plasmid
    #163536
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eA3A-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163536-plasmid-map-sequence-id-320176

    Enlarge

  19. GFP-2xPH-TAPP1 R211L mutant

    Plasmid
    #161991
    Purpose
    Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP
    Depositor
    David Bryant
    Publication
    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PH-TAPP1 (PLEKHA1 Human)
    Expression
    Mammalian
    Mutation
    R211L
    Available since
    Dec. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    161991-plasmid-map-sequence-id-318727

    Enlarge

  20. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid
    #154910
    Purpose
    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Publication
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phytochrome B (PHYB Mustard Weed, Synthetic)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB
    Promoter
    CMV
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154910-plasmid-map-sequence-id-318440

    Enlarge

Showing: 11881 - 11900 of 27462 results