34,175 results

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p3998 pGEX HHR23A

Plasmid

#10864

Depositor

Peter Howley

Publication

Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

Insert

HHR23A (RAD23A Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
SAP130

Plasmid

#11241

Depositor

Robin Reed

Publication

Das et al Mol Cell Biol. 1999 Oct . 19(10):6796-80

Insert

SAP130 (SF3B3 Human)

Expression

Bacterial

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LCC-ICCG_pET21b

Plasmid

#215408

Purpose

Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

LCC-ICCG

Expression

Bacterial

Mutation

F243I/D238C/S283C/Y127G

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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OaAEP1_C247A

Plasmid

#200307

Purpose

Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

Depositor

Artur Krężel

Publication

Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Tags

Smt3

Expression

Bacterial

Mutation

Cysteine 247 to Alanine

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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His-Tev-UbE1

Plasmid

#186804

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

Depositor

Lalit Deshmukh

Publication

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-UbE1 (UBA1 Human)

Tags

His6-Tev

Expression

Bacterial

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Publication

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

HIS-tag

Expression

Bacterial

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Publication

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pN251

Plasmid

#188471

Purpose

Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

Depositor

Le Cong

Publication

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

enCas12a-HF (gift from Dr. Keith Joung)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Publication

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBHM2403

Plasmid

#171687

Purpose

pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

Depositor

Hiten Madhani

Publication

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

CnoCAS9

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172503

Purpose

Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPSU15

Plasmid

#173828

Purpose

Expresses 15 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPAC

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

Plasmid

#160736

Purpose

Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Publication

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Publication

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCutamp

Plasmid

#140632

Purpose

Plasmid-curing in Escherichia coli by targeting the AmpR promoter

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter

Use

CRISPR

Expression

Bacterial

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCB578

Plasmid

#141095

Purpose

E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

Depositor

Chase Beisel

Publication

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Inserts

Cas9 and tracrRNA
repeat-spacer-repeat array

Expression

Bacterial

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXT130_TwinStrep-SUMO-ShTnsC

Plasmid

#135526

Purpose

Protein expression construct for ShTnsC

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsC

Tags

TwinStrep-SUMO

Expression

Bacterial

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Publication

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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