30,567 results

1. yTREX

   Plasmid

   #177306

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTlpA39-Wasabi

   Plasmid

   #86116

   Purpose

   mWasabi reporter gene gated by TlpA39

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA39
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A, D135V, A217V, L236F

   Promoter

   pTlpA and pTlpA, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPC1-FLAG-Synaptojanin 1-145

   Plasmid

   #22293

   Depositor

   Pietro De Camilli

   Article

   Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

   Insert

   Synaptojanin 1_145 (SYNJ1 Human)
   

   Use

   Tags

   Flag and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBSV2_PresT-mCherryBb

   Plasmid

   #118238

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   resT promoter driven mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSNAP-tag (T7) Vector

   Plasmid

   #101137

   Purpose

   Expression of protein of interest with SNAP-Tag in bacterial cells

   Depositor

   Ana Egana, New England Biolabs

   Type

   Empty backbone

   Use

   Tags

   SNAP-tag (SNAP26b)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLys-M1 backbone

   Plasmid

   #109386

   Purpose

   Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. S100A6

    Plasmid

    #196217

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses S100A6.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    S100A6 (S100A6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJCSUP35(1-253)

    Plasmid

    #1089

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 1 - 253 of SUP35

    Promoter

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pET11a-CA(A14C, E45C)

    Plasmid

    #79045

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    CA (A14C, E45C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A14C and E45C mutations in HIV-1 CA protein

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSV2_P0031-mCherryBb

    Plasmid

    #118240

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0031 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-CHMP4A (1-204) (pGEX2T(TEV))

    Plasmid

    #80632

    Purpose

    Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-204

    Promoter

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC101_TIMER

    Plasmid

    #103057

    Purpose

    low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest-565

    Plasmid

    #11520

    Purpose

    Gateway destination vector for bacterial expression; His,GST tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pComb3

    Plasmid

    #63888

    Purpose

    For phage display of antibody libraries by fusion to modified geneIII.

    Depositor

    Carlos Barbas

    Article

    Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

    Type

    Empty backbone

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. NRASA

    Plasmid

    #25256

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NRASA:M1-N172 (NRAS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids M1-N172

    Promoter

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. OPEN HLA-A*02:01-BirA

    Plasmid

    #215569

    Purpose

    E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    Glycine 120 to Cysteine

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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