31,436 results
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Plasmid#138412PurposeattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Att/phic31ExpressionBacterial and MammalianAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-IntNtr-eSrt2A
Plasmid#126015PurposeBacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-IntNtr-eSrt2A
Tags6xHis-SUMOExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.1
Plasmid#88994PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Cas12g1-noncoding
Plasmid#120880PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12g1
UseCRISPRExpressionBacterialPromoterlacAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG13-pBAD-TetRssrA
Plasmid#102449PurposeExpresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBFC0694
Plasmid#186693PurposeShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsB
tnsC
tniQ
Cas12k
Sh_lacZ_α_1 gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo
ExpressionBacterialPromoterPLacAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p_mFAP2b
Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
Tags6xHis-thrombin-TEVExpressionBacterialAvailable SinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_li_Tq2CFP
Plasmid#136971PurposeGoldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linkerDepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
KRAS2B
Plasmid#55655Purposeexpression of mouse KRAS2BDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only