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pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Article

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHB-4

Plasmid

#140957

Purpose

For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Ubiquitin G76C

Plasmid

#86583

Purpose

Bacterial expression of Ubiquitin G76C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; G76C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pET3a-hUBA1

Plasmid

#63571

Purpose

Expression of human UBA1 tagged with His

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBA1 (UBA1 Human)

Expression

Bacterial

Mutation

Isoform 2 (1018 amino acids)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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IRF3-V5

Plasmid

#169775

Purpose

Express IRF3 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)

Tags

V5 tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R

Plasmid

#58978

Purpose

expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMV762-Peredox-mCherry

Plasmid

#90217

Purpose

Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.

Depositor

Ashwani Kumar

Article

Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;

Insert

Codon Optimized Peredox-mCherry

Use

Shuttle vector

Expression

Bacterial

Promoter

groEL

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-iGluSnFR4f

Plasmid

#234444

Purpose

Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4f

Tags

6xHis-Xpress epi

Expression

Bacterial

Promoter

T7

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD68Kan2-AphA

Plasmid

#191248

Purpose

RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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