30,416 results

1. Alpha1

   Plasmid

   #118044

   Purpose

   GoldenBraid2.0 Alpha 1 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HMGCS1

   Plasmid

   #38844

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HMGCS1 (HMGCS1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCDB180

   Plasmid

   #80677

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Type

   Empty backbone

   Use

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. plRL118

   Plasmid

   #163632

   Purpose

   Renilla luciferase reporter plasmid; Giles integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mycobacterial Optimized Promoter (MOP)

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Omega 1

   Plasmid

   #118046

   Purpose

   GoldenBraid2.0 Omega 1 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV ie enhancer/promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

7. SRC_HUMAN_D0

   Plasmid

   #79700

   Purpose

   This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SRC (SRC Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOxyR_rfp

   Plasmid

   #194155

   Purpose

   Red Fluorescent Reporter of intracellular hydrogen peroxide

   Depositor

   Sven Panke

   Article

   Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

   Insert

   transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PoxyR, PoxyS

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18T-mini-Tn7T-Tp-gfpmut3

    Plasmid

    #65033

    Purpose

    TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge