30,416 results

1. EcAvs4

   Plasmid

   #188794

   Purpose

   Avs4 from Escherichia coli NCTC11132

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRaGE Pyl TAG GFP Y35TAG

   Plasmid

   #160041

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

   Inserts

   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   deGFP
   

   Use

   Synthetic Biology

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed tyrosine 35 to TAG stop-codon (Site numbe…

   Promoter

   P70b promoter and T500 terminator, Vibrio natrieg…

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDM067

   Plasmid

   #216820

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold2
   

   Use

   Tags

   VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18T-mini-Tn7T-Gm-eyfp

   Plasmid

   #65031

   Purpose

   GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet-T7-Ct-YhjH

   Plasmid

   #79167

   Purpose

   Dual expression of Ct biosensor and YhjH under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   yhjH
   T7-Spinach2-Ct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. YARS_pNIC-CTB10H

   Plasmid

   #153059

   Purpose

   Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   YARS (YARS1 Human)
   

   Use

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SNAP-23

   Plasmid

   #186686

   Purpose

   Expresses SNAP-23 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   SNAP23
   

   Use

   Tags

   GST and thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. HP17_KO7

   Plasmid

   #120346

   Purpose

   Helper plasmid for production of phagemid ssDNA, based on M13KO7

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Coding part of the M13KO7 helper phage genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   native phage promoters

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192789

   Purpose

   aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody minimizer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pALACE

    Plasmid

    #31853

    Depositor

    Yossef Av-Gay

    Article

    Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

    Type

    Empty backbone

    Use

    Escherichia coli-mycobacterium shuttle plasmid

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Acetamidase operone

    Available Since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only