30,416 results

1. EcRho

   Plasmid

   #113121

   Purpose

   Expresses E. coli Rho

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   Rho
   

   Use

   Tags

   MGH

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Omega 2

   Plasmid

   #118047

   Purpose

   GoldenBraid2.0 Omega 2 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Alpha2

   Plasmid

   #118045

   Purpose

   GoldenBraid2.0 Alpha 2 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FKBP1AA-c001

   Plasmid

   #53671

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   FKBP1AA
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Brg1 Del2 pET28a

   Plasmid

   #122116

   Purpose

   Expression Brg1 amino acids 1-837 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   6XHis and His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCryptDel4.8

   Plasmid

   #141293

   Purpose

   Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Inserts

   RelB
   gRNA targetting pMUT2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Native promoter from pMUT2 relB/relE operon and P…

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET3a-hUb

   Plasmid

   #61937

   Purpose

   Expression of hUb in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   human ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET21a-Streptavidin-Dead

   Plasmid

   #20859

   Purpose

   Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Dead
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Triple mutant of wt Streptavidin N23A,S27D,S45A

   Promoter

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTA-Mob 2.0

    Plasmid

    #149662

    Purpose

    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

    Depositor

    Bogumil Karas

    Article

    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge