30,416 results

1. EcAvs4

   Plasmid

   #188794

   Purpose

   Avs4 from Escherichia coli NCTC11132

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRaGE Pyl TAG GFP Y35TAG

   Plasmid

   #160041

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

   Inserts

   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   deGFP
   

   Use

   Synthetic Biology

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed tyrosine 35 to TAG stop-codon (Site numbe…

   Promoter

   P70b promoter and T500 terminator, Vibrio natrieg…

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAJM.773

   Plasmid

   #108527

   Purpose

   VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   VanRAM + PVanCC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbS5a-Opto-Cre-Vvd-2

   Plasmid

   #160400

   Purpose

   Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd-2
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet-T7-Ct-YhjH

   Plasmid

   #79167

   Purpose

   Dual expression of Ct biosensor and YhjH under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   yhjH
   T7-Spinach2-Ct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. YARS_pNIC-CTB10H

   Plasmid

   #153059

   Purpose

   Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   YARS (YARS1 Human)
   

   Use

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SNAP-23

   Plasmid

   #186686

   Purpose

   Expresses SNAP-23 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   SNAP23
   

   Use

   Tags

   GST and thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192789

   Purpose

   aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody minimizer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pALACE

   Plasmid

   #31853

   Depositor

   Yossef Av-Gay

   Article

   Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

   Type

   Empty backbone

   Use

   Escherichia coli-mycobacterium shuttle plasmid

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   Acetamidase operone

   Available Since

   July 9, 2012

   Availability

   Academic Institutions and Nonprofits only

10. EcRho

    Plasmid

    #113121

    Purpose

    Expresses E. coli Rho

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    Rho
    

    Use

    Tags

    MGH

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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