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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pVHD

Plasmid

#61303

Purpose

Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

Type

Empty backbone

Tags

HA

Expression

Bacterial

Promoter

PV10

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF_RT_FAST_RFP

Plasmid

#136979

Purpose

Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

Depositor

Klaus Theres

Article

Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

Insert

seed coat marker RFP

Expression

Bacterial

Mutation

BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-mGreenLantern

Plasmid

#161913

Purpose

mGreenLantern fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

6xHis

Expression

Bacterial

Promoter

araB

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCWU6-xylR-E-lepB

Plasmid

#190725

Purpose

fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-lepB

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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VN1852 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235118

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses ASf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1316 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_eGFP-wcKanR

Plasmid

#235113

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pWLlR5

Plasmid

#149492

Purpose

Broad-Host range expression vector, incW (pSa) origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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UbcH5c WT pET28a

Plasmid

#12643

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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ACS1

Plasmid

#13746

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Synthetase 1 (Acss2 Mouse)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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SoxS-GFP

Plasmid

#220165

Purpose

To track the natural SoxS promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

SoxS promoter only

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMAZ-SK

Plasmid

#73962

Purpose

self-killing gRNA plasmid pMAZ-SK

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Insert

gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLN-AIDA-Tyr1

Plasmid

#192831

Purpose

IPTG inducible AIDA-Tyrosinase expression

Depositor

André Studart

Article

Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

Insert

AIDA-Tyr1

Expression

Bacterial

Promoter

pL-lac

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pOPARA1

Plasmid

#200416

Purpose

Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)

Depositor

Kamil Witek

Article

Lesley et al bioRxiv

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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WT USP7 catalytic

Plasmid

#227201

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 207-560

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDM026

Plasmid

#216808

Purpose

Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Type

Empty backbone

Use

CRISPR; Fungal expression

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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