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33,441 results

Showing: 2561 - 2580 of 33441 results

  4. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Publication
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  5. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    112568-plasmid-map-sequence-id-217224

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  13. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Publication
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  14. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  15. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  18. pGBW-m4046979

    Plasmid
    #145611
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    145611-plasmid-map-sequence-id-292502

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Showing: 2561 - 2580 of 33441 results