30,577 results

1. PB-TRE3G-MYCN

   Plasmid

   #104542

   Purpose

   PiggyBac vector encoding doxycycline-inducible human MYCN

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM534

   Plasmid

   #112530

   Purpose

   pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Insert

   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL(HrtO)

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28c-F30-2xdBroccoli

   Plasmid

   #66843

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-ATG8(416)/GFP-AUT7(416)

   Plasmid

   #49425

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

   Depositor

   Daniel Klionsky

   Article

   Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ATG8

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

   Plasmid

   #199248

   Purpose

   Fluorescent labelling of bacterial cells with sfGFP

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized sfGFP gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC101-Donor

   Plasmid

   #140630

   Purpose

   Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   LE, RE, KanR, temperature sensitive pSC101 origin of replication
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetOminiCMV-OSKM

   Plasmid

   #136541

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor14-ShHELIX-KanR (CJT70)

   Plasmid

   #181793

   Purpose

   pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRECMYC-Ebu1

    Plasmid

    #240713

    Purpose

    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

    Depositor

    Seth Shipman

    Insert

    Ebu1 RT, Eco1 ncRNA, MspRecT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAK184.tetR.PT5-3.dCas9

    Plasmid

    #232482

    Purpose

    optimized ahTet inducible dCas9 for insertion into C. difficile genome

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Insert

    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-PT5-3

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJC.15A.sgRNA.TA

    Plasmid

    #232483

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Inserts

    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 constitutive promoter and native promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-LIC-N8C_Fab2b

    Plasmid

    #203692

    Purpose

    Expresses N8C_Fab2b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pE5770

    Plasmid

    #170777

    Purpose

    expression of hybrid proteins in E. coli

    Depositor

    Anthony Schryvers

    Article

    Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

    Insert

    Kan resistance (kanR )
    

    Use

    Tags

    polyhisbioMbpTEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTK072

    Plasmid

    #65179

    Purpose

    Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConRE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSR358.4

    Plasmid

    #125114

    Purpose

    Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-liaR(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30-His-IF1

    Plasmid

    #124123

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 1 (infA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T1 human PP2A Aα full length

    Plasmid

    #132634

    Purpose

    expresses human PP2A Aα full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A Aα (PPP2R1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p005-RFP-strong

    Plasmid

    #108317

    Purpose

    Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    RFP under controlf of strong synthetic constitutive promoter pFAB4005
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4005

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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