32,870 results
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Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
TagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT201
Plasmid#134878PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD
Plasmid#171238PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT004-LaG30VB
Plasmid#163384Purpose2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN systemDepositorInsertLaG30-VEEGFRtm-BFP (LaG30VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iSeroSnFR
Plasmid#128483PurposeFluorescent reporter for serotonin (bacterial expression)DepositorInsertiSeroSnFR
TagsHis, T7, and cpsfGFPExpressionBacterialPromoterT7Available SinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationWTPromoterT7Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E2D1
Plasmid#161606PurposeBacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formationDepositorInsertMSP1E2D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-P2A-EGFP_mRFPstuf
Plasmid#137730PurposeExpresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNADepositorTypeEmpty backboneUseCRISPR, Lentiviral, Mouse Targeting, and Syntheti…ExpressionBacterial and MammalianAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP089
Plasmid#158796PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-3
ExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD451-pA-APEX2
Plasmid#157863PurposeBacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labelingDepositorInsertpA-APEX2
ExpressionBacterialAvailable SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCL149
Plasmid#134457PurposeCmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.DepositorInsertPara-popZ-rbs-H3H4-msfGFPN-CTM.
ExpressionBacterialPromoterParaAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialPromoterT7Available SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry
Plasmid#133320PurposeBacterial expression of TDP43 with WT CTDDepositorAvailable SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIgnaviCas9
Plasmid#127595PurposeExpresses IgnaviCas9 in E. coliDepositorInsertIgnaviCas9
Tags6xHis Tag and Maltose binding proteinExpressionBacterialPromoterT7 promoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only