32,387 results
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Plasmid#235150PurposeBacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertHaloTag linked to SpyCatcher3
TagsHisExpressionBacterialPromoterT7Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
Tags6xhisExpressionBacterialPromoterT7 promoterAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
RACGAP1
Plasmid#38807PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxR
Plasmid#193625PurposeConstitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.DepositorInsertLuxR
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3b
Plasmid#203685PurposeExpresses N8C_Fab3bDepositorInsertN8C_Fab3b
ExpressionBacterialAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-Cdk5
Plasmid#24894DepositorAvailable SinceMay 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-E2Crimson
Plasmid#78478PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
ExpressionBacterialAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
ExpressionBacterialPromoterNAAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbB5k-GFP
Plasmid#35354DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
GST-Hsp90 N(9-236)
Plasmid#22481DepositorAvailable SinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
ExpressionBacterialPromoterT7Available SinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
SQ171/pRibo-T
Plasmid#69342PurposeSQ171 strain supported by pRibo-T plasmidDepositorInsertRibo-T
ExpressionBacterialAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
ExpressionBacterialAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
ExpressionBacterialPromoterT7Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
ExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Bcatfl
Plasmid#24193DepositorAvailable SinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pG1K
Plasmid#71741PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianPromoterTRE3GVAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-myc
Plasmid#218191PurposeBacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
Tags2xmyc and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized for E. coli, residues 22-266PromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only