30,416 results

1. pET28a-cMSPdH5

   Plasmid

   #199771

   Purpose

   Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH5
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Promoter

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAD39_mIRG1_4-462_pvp008

   Plasmid

   #124873

   Purpose

   Expresses mouse CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (Acod1 Mouse)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 463-end.

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQTEV-HSPB8

   Plasmid

   #34710

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   heat shock 22 kDa protein 8 (HSPB8 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTAMAHISTEV_PfeA

   Plasmid

   #128943

   Purpose

   expresses PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Use

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75)

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET20b_Pa-PirA

   Plasmid

   #128956

   Purpose

   expresses Pseudomonas aeruginosa PirA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1 p37

   Plasmid

   #169015

   Purpose

   Expresses GST-p37 (human, codon optimized for expression in bacteria)

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   p37 (UBXN2B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   sequence was codon optimized for expression in E.…

   Promoter

   tac

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX4T-1-6AlanineHE1

   Plasmid

   #165098

   Purpose

   bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1

   Depositor

   Arthur Haas

   Insert

   Uba1 (UBA1 Human)
   

   Use

   Tags

   GST-6-alanine

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTXB3-Flag PLK1 T210D

    Plasmid

    #68272

    Purpose

    bacterial expression of Plk1 T210D mutant

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    T210D

    Promoter

    T7

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only