29,729 results

1. OA-1136J

   Plasmid

   #153023

   Purpose

   Express His-MBP-TEV-CasRx recombinant protein

   Depositor

   Omar Akbari

   Article

   Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

   Insert

   CasRx
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-KSRP

   Plasmid

   #23019

   Depositor

   Douglas Black

   Article

   Min et al Genes Dev. 1997 Apr 15. 11(8):1023-36.

   Insert

   KSRP (KHSRP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT9679-sgRNA-BFP

   Plasmid

   #162988

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM5409

   Plasmid

   #132662

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PaadA and PconII

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYTK-DN1

   Plasmid

   #180282

   Purpose

   Cas9 cassette (TU1)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS - pPGK1 - Cas9 - tPGK1 - ConR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pPGK1

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAKgfp1

   Plasmid

   #14076

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138129

   Purpose

   Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-6His-SUMO-Nsp10

   Plasmid

   #154959

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pD451-pA-APEX2

   Plasmid

   #157863

   Purpose

   Bacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labeling

   Depositor

   Alex Kentsis

   Insert

   pA-APEX2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAN1717

    Plasmid

    #74696

    Purpose

    RPU standard plasmid

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Insulated J23101-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLRD14

    Plasmid

    #208266

    Purpose

    Production of syn-pimara-7,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    osCPS4
    osKSL4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTHSSe_42

    Plasmid

    #109240

    Purpose

    pSC101 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_BTK-1/1

    Plasmid

    #91350

    Purpose

    Protein expression and purification of human SH3 domain construct BTK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BTK-1/1 (BTK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MSP1D1deltaH4H5

    Plasmid

    #71716

    Purpose

    Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIOX

    Plasmid

    #51300

    Purpose

    E.coli expression vector for His8-SUMO tagged recombinant protein

    Depositor

    Andrea Mattevi

    Type

    Empty backbone

    Use

    Tags

    His8 + SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMG-AIS

    Plasmid

    #225133

    Purpose

    For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMAK464

    Plasmid

    #140702

    Purpose

    NBαEGFP

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB’

    Plasmid

    #162703

    Purpose

    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5513T>G (tetR E37A); 7758G>T (second tet op…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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