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31,059 results

Showing: 3041 - 3060 of 31059 results

  1. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  4. HK2

    Plasmid
    #25529
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HK2 (HK2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pSEVA3b67Rb

    Plasmid
    #58311
    Purpose
    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    mRFP1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58311-plasmid-map-sequence-id-89824

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  14. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available Since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

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  16. pHJL3

    Plasmid
    #199378
    Purpose
    Encodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7s::SL2::mScarlet-I
    Expression
    Bacterial
    Mutation
    no mutations
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199378-plasmid-map-sequence-id-391040

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Showing: 3041 - 3060 of 31059 results