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pMRE-Himar-155

Plasmid

#185939

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-157

Plasmid

#185940

Purpose

Himar transposon delivery plasmid that delivers a green fluorescent mClover3 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mClover3

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-143

Plasmid

#185932

Purpose

Himar transposon delivery plasmid that delivers a yellow fluorescent sYFP2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

sYFP2

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 epsilon GST

Plasmid

#13279

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 epsilon (Ywhae Rat)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTnsABC

Plasmid

#160031

Purpose

Expresses V. cholerae TnsABC

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Mutation

no

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJV53-Cas12a

Plasmid

#158706

Purpose

CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

FnCpf1

Use

CRISPR

Expression

Bacterial

Promoter

TetO

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO AMPKa (11-281)

Plasmid

#177851

Purpose

Bacterial Expression of AMPKa1

Depositor

Karsten Melcher

Article

Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

Insert

AMPK a1 (PRKAA1 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

P438

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-iRFP

Plasmid

#190182

Purpose

Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
iRFP
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmApple

Plasmid

#191259

Purpose

Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

Depositor

Robert Campbell

Article

Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

Insert

LSSmApple

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBb(RK2)1k-GFPuv

Plasmid

#106394

Purpose

IPTG-inducible trc promoter expressing gfpuv on RK2 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pREDKI

Plasmid

#51626

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5Knob

Plasmid

#198289

Purpose

Produces PVCs retargeted with knob domain from hAd5 virus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5Knob

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-Oct4

Plasmid

#16352

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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