30,744 results

1. pJMP1337

   Plasmid

   #119270

   Purpose

   for cloning new sgRNAs

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAN1201

   Plasmid

   #74695

   Purpose

   Circuit backbone plasmid (LacZa insert)

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-ADARcd-E488Q-V5

   Plasmid

   #139686

   Purpose

   Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Adenosine deaminase acting on RNA (Adar Fly)
   

   Tags

   V5

   Expression

   Bacterial and Insect

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM1s3TsR

   Plasmid

   #137922

   Purpose

   E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSetB-KanR-LiLac

   Plasmid

   #184569

   Purpose

   Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

   Depositor

   Gary Yellen

   Article

   Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

   Insert

   LiLac fluorescent sensor of lactate
   

   Tags

   His7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad33_ISTfu1_reRNA

   Plasmid

   #205355

   Purpose

   Bacterial expression of ISTfu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISTfu1 TnpB
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. NNMT

   Plasmid

   #40734

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NNMT (NNMT Human)
   

   Tags

   His Thrombin

   Expression

   Bacterial

   Mutation

   K100A:E101A:E103A

   Available Since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pCas9CyMutL

   Plasmid

   #166981

   Purpose

   Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Inserts

   MutL (mutL )
   CymR
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   pCymRC and pLacI

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_CDC45_dTAG

   Plasmid

   #232733

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459_MCM2_sgRNA

    Plasmid

    #232730

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459_GINS4_gRNA

    Plasmid

    #232734

    Purpose

    Generates GINS4 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    GINS4 (GINS4 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19_GINS4_dTAG

    Plasmid

    #232735

    Purpose

    Generates GINS4 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    GINS4 (GINS4 Human)
    

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19_MCM2_dTAG

    Plasmid

    #232731

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. lacI-PT5-gusA-pBAV1k

    Plasmid

    #30501

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucuronidase (gusA Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDG04163

    Plasmid

    #216373

    Purpose

    xhNup214-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup214-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-scSUMO

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS mouse ER81 [TJ#197]

    Plasmid

    #16282

    Depositor

    Thomas Jessell

    Insert

    ER81 (Etv1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pSR358.4

    Plasmid

    #125114

    Purpose

    Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-liaR(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSDM177

    Plasmid

    #194476

    Purpose

    pHERD30T-crRNA(Lse)_V3

    Depositor

    Joseph Bondy-Denomy

    Article

    Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

    Insert

    crRNA(Lse)
    

    Expression

    Bacterial

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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