31,059 results
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pQTEV-HSPB8
Plasmid#34710DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-SMAD4
Plasmid#16491DepositorAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTCF5 HLA-A*01:01
Plasmid#180448PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*01:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXT128_TwinStrep-SUMO-ShCas12k
Plasmid#135524PurposeProtein expression construct for ShCast12kDepositorInsertShCas12k
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-LAC
Plasmid#64965Purposemini-Tn7 vector with Ptac expression cassetteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
6xHis-MBP-huBsCas12a
Plasmid#174671PurposeExpresses 6xHis-MBP tagged BsCas12aDepositorArticleInserthuBsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…ExpressionBacterialPromoterT7Available SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2B-58
Plasmid#177928PurposeExpresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)DepositorArticleInsertEGFP
ExpressionBacterial and YeastPromoterTET4 (contains four rtTA binding sites)Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-std
Plasmid#109417PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB076i7
Plasmid#79201PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL3
Plasmid#67376PurposeExpression of native hArl3 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET YBX-1
Plasmid#13035DepositorAvailable SinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET20b_Pa-PiuA
Plasmid#128955Purposeexpresses Pseudomonas aeruginosa PiuA in E. coliDepositorInsertiron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
Tags6xHis and pelB leaderExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
ExpressionBacterialPromoterAraC-ParaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA
Plasmid#110203PurposeRecipient vector for Cranial Neural Crest-specific expression of protein of interestDepositorInsertenhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
TagseGFPExpressionBacterialAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only