33,441 results
-
Plasmid#174500Purposebacterial co-expression of human SEPT7 and of human SEPT9_i1DepositorAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
bdSUMO-GFP-HiBit
Plasmid#170989PurposeFor bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-HA-AR-NTD-DBD
Plasmid#171236PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETDuet-1 TIM9,10
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertExpressionBacterialPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-TK-Firefly-Control
Plasmid#122277PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionBacterialPromoterThymidine kinaseAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-4T-2/Rabaptin-5:R5BD
Plasmid#168808PurposeProduction of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily membersDepositorAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCJ189
Plasmid#162666PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLG014 (RADAR)
Plasmid#157892PurposeExpresses the RADAR defense systemDepositorInsertRADAR
ExpressionBacterialAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mu-GM-CSF-pCIneo
Plasmid#163518PurposeExpresses murine GM-CSF in mammalian cellsDepositorInsertmurine GM-CSF
ExpressionBacterial and MammalianAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC2085
Plasmid#158721PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
ExpressionBacterialPromotercLac143Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
ExpressionBacterialAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLG033 (ietAS)
Plasmid#157911PurposeExpresses the ietAS defense systemDepositorInsertietAS (ATPase + serine protease)
ExpressionBacterialAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG026 (tmn)
Plasmid#157904PurposeExpresses the tmn defense systemDepositorInserttmn (transmembrane ATPase)
ExpressionBacterialAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorExpressionBacterialPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL3-pCMV7.1
Plasmid#155021PurposeN-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vectorDepositorAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
Tagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MazF-WT
Plasmid#155202PurposeTo express recombinant MazF protein.DepositorInsertMazF
ExpressionBacterialAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only