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Showing: 3421 - 3440 of 29646 results

  1. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  2. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  3. AK1

    Plasmid
    #39134
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK1 (AK1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pFA6a-PAmCherry-KanMX6

    Plasmid
    #87026
    Purpose
    Tag S. pombe genes in C terminal with PAmCherry
    Depositor
    Julien Berro
    Insert
    PAmCherry
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87026-plasmid-map-sequence-id-172997

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  9. pEXP5-NT/pLux eGFP

    Plasmid
    #193624
    Purpose
    Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    eGFP
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    pLux
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193624-plasmid-map-sequence-id-381997

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  10. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

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  13. HK2

    Plasmid
    #25529
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HK2 (HK2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. AK3L1

    Plasmid
    #39135
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK3L1 (AK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 and VanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  17. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Use
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

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  20. AK5

    Plasmid
    #39136
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK5 (AK5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 3421 - 3440 of 29646 results