30,744 results

1. pRSET his-eGFP

   Plasmid

   #113551

   Purpose

   For bacterial expression and purification of eGFP

   Depositor

   Jeanne Stachowiak

   Article

   Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

   Insert

   his-eGFP
   

   Expression

   Bacterial

   Mutation

   A206K

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTP434

   Plasmid

   #104167

   Purpose

   Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
   

   Tags

   14x Histidine tag and NEDD8 from Brachypodium dis…

   Expression

   Bacterial

   Mutation

   3x Cysteines located at bps 493-495, 520-522, and…

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SENP1 (catalytic domain)

   Plasmid

   #16356

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP1 catalytic domain (SENP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 419 to 644 catalytic domain of human S…

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK CA RhoA

   Plasmid

   #112894

   Purpose

   promoter-inducible expression of constitutively-active RhoA

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   CA RhoA (RHOA Human)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5068 pGEX-6P-1 Brd4 full-length

   Plasmid

   #14447

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (BRD4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. SARS-CoV-2_N_FL

   Plasmid

   #157867

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLAG-XBP1u-FLuc

   Plasmid

   #31239

   Depositor

   Jimtong Horng

   Article

   Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

   Insert

   FLAG-XBP1u-FLuc (XBP1 Human)
   

   Tags

   Flag and Fluc

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. CMV-FlicR1

   Plasmid

   #74142

   Purpose

   Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

   Depositor

   Robert Campbell

   Article

   Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

   Insert

   FlicR1
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQFmcs-2H12.D11

   Plasmid

   #221183

   Purpose

   cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2
   

   Expression

   Bacterial

   Promoter

   PQ5 (cumate-inducible)

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18T-mini-Tn7T-hph-Ptet1

    Plasmid

    #218367

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTOX1

    Plasmid

    #127446

    Purpose

    Encodes YhaV toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    yhaV toxin
    

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_T7-ARG2

    Plasmid

    #106474

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p405MET25

    Plasmid

    #15961

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28C-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204402

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AzoRS-4

    Plasmid

    #182884

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

    Insert

    AzoRS-4
    

    Expression

    Bacterial

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRFP-human APPL1

    Plasmid

    #22202

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRuby2-pBAD

    Plasmid

    #54771

    Purpose

    Localization: Protein Expression Vector , Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    6xHis and mRuby2

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMOD3-proD/Lux-nat

    Plasmid

    #120324

    Purpose

    Bacterial luminescence

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    Lux operon
    

    Expression

    Bacterial

    Promoter

    proD

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_His6-SUMO-TEV-TtCsm6

    Plasmid

    #172486

    Purpose

    Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
    

    Tags

    His-SUMO-TEV

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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