29,729 results

1. pETM33_Nsp4

   Plasmid

   #156474

   Purpose

   Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp4 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRMCD28

   Plasmid

   #167556

   Purpose

   Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

   Type

   Empty backbone

   Use

   Tags

   PhoA'

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMyBADNT-kan

   Plasmid

   #84691

   Purpose

   Protein expression in mycobacteria

   Depositor

   Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   AraC

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-Hook3 aa 1-552

   Plasmid

   #74614

   Purpose

   Expresses a truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-552 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFNF

   Plasmid

   #22687

   Depositor

   Robert Benezra

   Article

   Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

   Insert

   Neo*
   

   Use

   Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   FRT-pPGK-pEM7-Neo*-bpA-FRT

   Promoter

   Available since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

   Plasmid

   #159562

   Purpose

   E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)
   

   Use

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Mutation

   G12D

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

   Plasmid

   #177850

   Purpose

   Bacterial Expression of AMPK

   Depositor

   Karsten Melcher

   Article

   Yan et al Science. 2021 Jul 23;373(6553):413-419.

   Insert

   AMPK (a1b2g1)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-iNOS

   Plasmid

   #19295

   Depositor

   Charles Lowenstein

   Article

   Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

   Insert

   inducible nitric oxide synthase (Nos2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ACACA

   Plasmid

   #39083

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACACA (ACACA Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-GST-AGO2

    Plasmid

    #24317

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    human AGO2 (AGO2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCVD364

    Plasmid

    #63739

    Purpose

    V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

    Depositor

    James Kaper

    Article

    Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

    Insert

    nanH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. p324_DG

    Plasmid

    #234690

    Purpose

    Golden gate domesticated pET28b with TEV-His6

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    TEV-His6

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p370_DG

    Plasmid

    #234693

    Purpose

    Golden gate domesticated pET28b with 3C-mNeongreen-His10

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    3C-mNeongreen-His10

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p277_DG

    Plasmid

    #234695

    Purpose

    Golden gate domesticated pET28b for scarless cloning with His6-3C

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p357_DG

    Plasmid

    #234700

    Purpose

    Golden gate domesticated pET28b for scarless cloning with His10-GST-3C

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-GST-3C

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p368_DG

    Plasmid

    #234691

    Purpose

    Golden gate domesticated pET28b with 3C-His6

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    3C-His6

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p349_DG

    Plasmid

    #234679

    Purpose

    Golden gate domesticated pET28b with His10-GST-3C

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-GST-3C

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p351_DG

    Plasmid

    #234681

    Purpose

    Golden gate domesticated pET28b with His10-mNeongreen-3C

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-mNeongreen-3C

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge