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34,213 results

Showing: 3941 - 3960 of 34213 results

  2. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  3. pFP.E227

    Plasmid
    #138249
    Purpose
    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P17_up1691, P20_992, and iP16_3622
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138249-plasmid-map-sequence-id-273529

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  5. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Chris Barnes
    Publication
    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    133307-plasmid-map-sequence-id-272413

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  6. pGGF_RT_FAST_Venus

    Plasmid
    #136980
    Purpose
    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence
    Depositor
    Klaus Theres
    Insert
    seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    Expression
    Bacterial
    Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136980-plasmid-map-sequence-id-269080

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  7. KE-005

    Plasmid
    #133440
    Purpose
    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4A1 (EIF4A1 Human)
    Expression
    Bacterial
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133440-plasmid-map-sequence-id-267708

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  8. pSLC-255

    Plasmid
    #124292
    Purpose
    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Publication
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124292-plasmid-map-sequence-id-243084

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  9. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Publication
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

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  10. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid
    #110555
    Purpose
    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.
    Depositor
    Martin Pule
    Publication
    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg
    Insert
    Anti-RD114 heavy chain
    Tags
    mIgG2A
    Expression
    Bacterial and Mammalian
    Promoter
    EF-1 alpha core promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110555-plasmid-map-sequence-id-212943

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  16. SiriusGFP

    Plasmid
    #123201
    Purpose
    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.
    Depositor
    Joseph Santos-Sacchi
    Publication
    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SiriusGFP
    Use
    Mouse Targeting
    Tags
    N/A
    Expression
    Bacterial and Mammalian
    Mutation
    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    123201-plasmid-map-sequence-id-239192

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  19. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Publication
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122174-plasmid-map-sequence-id-237469

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Showing: 3941 - 3960 of 34213 results