34,213 results

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pC012 LwCas13a locus with nontargeting spacer

Plasmid

#91904

Purpose

LwCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with non-targeting spacer

Use

CRISPR

Expression

Bacterial

Available since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pRM006

Plasmid

#98922

Purpose

Express Histag-SUMO-FtsZ for purification in E coli

Depositor

Jie Xiao

Publication

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsZ

Tags

Histag-SUMO

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Publication

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBZcsx27

Plasmid

#89900

Purpose

Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

Depositor

Feng Zhang

Publication

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

csx27

Expression

Bacterial

Promoter

Lac

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKD182

Plasmid

#90952

Purpose

Expresses ThsS under the Ptac promoter

Depositor

Jeffrey Tabor

Publication

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Ptac

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pccGFPG83I

Plasmid

#73650

Purpose

Fluorescent reporter for transmembrane protein dimerization negative control.

Depositor

Alessandro Senes

Publication

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Insert

glycophorin A- G83I

Expression

Bacterial

Mutation

glycine 83 to isoleucine

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLinker-BirA-3xHA-HXGPRT-loxP

Plasmid

#86668

Purpose

for generation of BirA tagged strains using CRISPR tagging technology

Depositor

David Sibley

Insert

Linker-BirA-3xHA-HXGPRT 3'UTR

Expression

Bacterial

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBS_flGAD

Plasmid

#87031

Purpose

Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria

Depositor

Janice Blum

Insert

flGAD (GAD2 Human)

Expression

Bacterial

Promoter

Lac-Z

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET14-GFP15_Encap

Plasmid

#86407

Purpose

Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Publication

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 15aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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CeNL_pRSETb

Plasmid

#89510

Purpose

Cyan color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMycoFos

Plasmid

#84577

Purpose

Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

Depositor

Nicholas Coleman

Publication

Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

Type

Empty backbone

Expression

Bacterial

Available since

April 7, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Publication

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Expression

Bacterial

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
pTriex-mVenus-PI-RhoA(Q63L)

Plasmid

#86978

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Publication

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

LOV2
RhoA (RHOA Human)

Tags

6xHis and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Publication

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-EPN-24

Plasmid

#79939

Purpose

PH-Late1-I3-01-myc

Depositor

Neil King

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EPN-24

Expression

Bacterial and Mammalian

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pUS116

Plasmid

#84578

Purpose

Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.

Depositor

Nicholas Coleman

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRAM18dRGA[MCS]

Plasmid

#84683

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Publication

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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SPS-6803

Plasmid

#80458

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

Depositor

Daniel Ducat

Publication

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET30-2-PSPH

Plasmid

#83909

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine phosphatase (PSPH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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