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pGMβ1

Plasmid

#216202

Purpose

Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

Depositor

Yasuko Sasaki

Article

Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

Type

Empty backbone

Use

Shuttle vector, conjugal plasmid, theta type rep…

Expression

Bacterial

Promoter

lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

Available Since

Jan. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDL17

Plasmid

#106483

Purpose

pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
exo
lacI
rhaR
rhaS

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-33

Plasmid

#214342

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL33 (IL33 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-StefinA

Plasmid

#182419

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_Cre

Plasmid

#201198

Purpose

AAV vector for cre expression under EF1 promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

cre
EF1alpha promoter

Use

AAV

Expression

Bacterial and Mammalian

Mutation

nuclear targeting cre

Promoter

N/A and hEF1alpha promoter

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

Plasmid

#241423

Purpose

Protein Transposition Cassette Containing CatN and CfaC

Depositor

Thomas Muir

Article

Hua et al Science. 2025 Apr 4;388(6742):68-74. doi

Insert

His5-Sumo-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

Tags

His5, HA

Expression

Bacterial

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC-prk:nptII

Plasmid

#239580

Purpose

Selection plasmid for Rubisco directed evolution

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PRK-NPTII

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 3xFLAG COP1

Plasmid

#237334

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

COP1 (COP1 Human)

Expression

Bacterial

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 dUBA (1-389)

Plasmid

#210426

Purpose

Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL dUBA (1-389) (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAV104

Plasmid

#63194

Purpose

pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET12a-PAPI

Plasmid

#19961

Depositor

Jonathan Tait

Article

Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Insert

Annexin V (ANXA5 Human)

Expression

Bacterial

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_35bpLinker

Plasmid

#157790

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_35bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK1126

Plasmid

#236187

Purpose

BsaI compatible gentamicin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

gentamicin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEERM6 Km

Plasmid

#64027

Purpose

Plasmid for integration into sqs site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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NLS-SpCas9mSA-His

Plasmid

#182951

Purpose

Express SpCas9mSA in bacteria

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

NLS-SpCas9mSA-His

Use

CRISPR

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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