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pAD-KPS12-Pman

Plasmid

#133832

Purpose

Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

mKate(KPS12)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pman

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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mu-IFNg-pCIneo

Plasmid

#163517

Purpose

Expresses murine IFN gamma in mammalian cells

Depositor

Thomas Weber

Insert

murine-IFN gamma (Ifng Mouse)

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-zif268

Plasmid

#18045

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

zif268

Expression

Bacterial

Mutation

Zif268 is fused to the omega subunit of RNA polym…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pGTVL2-CRBN-TBD

Plasmid

#196369

Purpose

protein expression of the GST-tagged Cereblon Thalidomide binding domain

Depositor

Stefan Knapp

Insert

CRBN (CRBN Human)

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

contains only T319-T425

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNCS mRFP

Plasmid

#91765

Purpose

Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

Depositor

Erik Rodriguez, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Insert

monomeric Red Fluorescent Protein1

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAJM.715

Plasmid

#108516

Purpose

PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTac-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET21b-Casp1-His

Plasmid

#11809

Depositor

Guy Salvesen

Insert

Caspase-1 (CASP1 Human)

Tags

His

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pOSIP-KT (KanR, P21)

Plasmid

#45987

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S2

Plasmid

#128590

Purpose

Expresses tagless S2 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S2 (rpsB E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-mEmerald

Plasmid

#103982

Purpose

Plasmid encoding mEmerald

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEmerald

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B-PP1alpha

Plasmid

#51768

Purpose

Expresses PP1 alpha residues 7-330

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

pp1 alpha

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 alpha residues 7-330

Promoter

T7-lac

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pET19b-EcMscL-TEV-mEGFP

Plasmid

#165097

Purpose

Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

Depositor

Neha Kamat

Article

Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

Insert

E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)

Use

Synthetic Biology

Tags

monomeric Enhanced Green Fluorescent Protein

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMM656

Plasmid

#68887

Purpose

Rhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSKBAD2

Plasmid

#15335

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein

Tags

GB1

Expression

Bacterial

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pGGB_RT_Tq2CFP_li

Plasmid

#136965

Purpose

Goldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linker

Depositor

Klaus Theres

Insert

Tq2CFP

Expression

Bacterial

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Article

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Expression

Bacterial

Promoter

tac

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRIM3-rfp

Plasmid

#74073

Purpose

GentR version of pRIM2 carrying rfp

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

rfp

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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