32,401 results
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Plasmid#138484PurposeExpresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteriaDepositorTags6xHis and GSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only
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His6_BcLOV4_Q355N_mCherry_BamUK
Plasmid#119762PurposeEncodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cellDepositorInsertBcLOV4-Q355N_mCherry
TagsmCherryExpressionBacterialMutationMutated glutamine at position 355 to asparaginePromoterT7Available SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBY1_FnCpf1cg
Plasmid#104622PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationcodon-optimized C.glutamicumPromoterhsp60Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS7
Plasmid#32056PurposeVector optimized for constitutive gene expression in Burkholderia genus and related bacteriaDepositorTypeEmpty backboneExpressionBacterialPromoterPs7 promoter, originated from Paraburkholderia xe…Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
TagsHisExpressionBacterialMutationWhole proteinPromoterT7Available SinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII-StrepII TEV LIC cloning vector (2RRT)
Plasmid#55215DepositorTypeEmpty backboneTags2xStrep II and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p47PX(aa2-132)
Plasmid#19007DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
ExpressionBacterialAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23B
Plasmid#201457PurposeExpresses human RAD23B with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTUB1*
Plasmid#65441PurposeExpresses activated OTUB1 in E. coliDepositorTagsHis-3CExpressionBacterialMutationActive site cysteine mutated to alaninePromoterT7Available SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Uricase
Plasmid#182417PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusionDepositorInsertVNp-mNeongreen-uricase
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_2
Plasmid#60574Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_2
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbA
Plasmid#112562PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-DsbA(AA20-208) and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW334i
Plasmid#237319Purposeexpresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1DepositorInsertA. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1
ExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
HSD11B1
Plasmid#39155PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E2D1
Plasmid#161606PurposeBacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formationDepositorInsertMSP1E2D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
ExpressionBacterialPromotercptAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBDC
Plasmid#135188PurposeIntegration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.DepositorInsertCmR
UseSynthetic BiologyExpressionBacterialMutationNonePromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNHA7
Plasmid#223649PurposebirA protein purificationDepositorInsertBioID_G118R
Tags6HisExpressionBacterialMutationG118RPromoterLacIAvailable SinceOct. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits