29,771 results

1. pH6HTC_PTBP1-Halo

   Plasmid

   #175302

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PTBP1 (PTBP1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD57

   Plasmid

   #119882

   Purpose

   Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_GFP+
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCBB-oriT

   Plasmid

   #32556

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTB-3

   Plasmid

   #22819

   Depositor

   Ryan Gill

   Article

   Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. TS246_pNKI-2-13_HALO-DPN7

   Plasmid

   #200720

   Purpose

   producing Methyl Tracer HALO protein in bacteria

   Depositor

   Bas van Steensel

   Article

   van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

   Insert

   HALO-DPN7
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWUKI 1+2

   Plasmid

   #79879

   Purpose

   Encodes Cas1+2 and a CRISPR array

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1
   Cas2
   CRISPR Array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTEV5_ RpMatB_1

   Plasmid

   #78205

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

   Insert

   matB (His6 C106A R286C Q457C K488A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C Q457C K488A

   Promoter

   T7

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBbA6c-RFP

   Plasmid

   #35290

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTM_COV2_NSP5_004_SUMO

   Plasmid

   #190062

   Purpose

   Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   3CLpro (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac

   Available since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMXs-Runx1

    Plasmid

    #131609

    Purpose

    expression of mouse Runx1 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 MBP TEV HDAC8_374

    Plasmid

    #122174

    Purpose

    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

    Depositor

    David Christianson

    Article

    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

    Insert

    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    

    Use

    Tags

    hexahistidine tag and tobacco etch virus protease…

    Expression

    Bacterial

    Mutation

    Residues Met1–Pro7 and H375-V377 have been delete…

    Promoter

    T7

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE6n

    Plasmid

    #17460

    Purpose

    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    EYFP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-eLACCO2.1

    Plasmid

    #208017

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC18T-mini-Tn7T-Gm-Pc-mCherry

    Plasmid

    #199247

    Purpose

    Fluorescent labelling of bacterial cells with mCherry

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized mCherry gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI

    Plasmid

    #134356

    Purpose

    Accessory plasmid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Gene VI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NXR1A

    Plasmid

    #42430

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NXR1A (NUDT18 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

    Plasmid

    #60815

    Purpose

    dCas9 with single cysteine residue (M1C) for site-specific labelling

    Depositor

    Jennifer Doudna

    Article

    O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

    Insert

    dCas9 M1C D10A C80S H840A C574S
    

    Use

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    M1C D10A C80S H840A C574S

    Promoter

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTCF6 HLA-A*24:02

    Plasmid

    #180445

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*24:02
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge