30,756 results

1. pQE80L Hu IL-15-His tag

   Plasmid

   #183256

   Purpose

   Bacterial expression of human IL-15 with N-terminal His tag

   Depositor

   Alexander McLellan

   Insert

   IL-15 (IL15 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDuet TDP-43 M337V

   Plasmid

   #27464

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Expression

   Bacterial

   Mutation

   Methionine 337 mutated to Valine (M337V)

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

   Plasmid

   #162576

   Purpose

   Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

   Depositor

   George Church

   Article

   Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

   Inserts

   Lambda Red-Beta
   E. coli SSB
   EcMutLE32K
   

   Expression

   Bacterial

   Mutation

   E32K mutated to give dominant negative phenotype

   Promoter

   pBAD

   Available Since

   May 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIC28-BSA4-G-L11

   Plasmid

   #127830

   Purpose

   Production of recombinant human ribosomal protein L11

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPL11 (RPL11 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM2314-RbcS-mNG

   Plasmid

   #165582

   Purpose

   Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

   Depositor

   Daniel Ducat

   Article

   Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Promoter

   Prbcs

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

   Plasmid

   #115795

   Purpose

   Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrIIA4
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EF-1a promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-omega

   Plasmid

   #114632

   Purpose

   Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

   Depositor

   Anushree Chatterjee

   Article

   Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

   Insert

   dCas9-omega
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet promoter

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRAV26-2

    Plasmid

    #123399

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV26-2 (TRAV26-2 Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RCAS(B)-Rheb-Q64L

    Plasmid

    #21050

    Depositor

    Peter Vogt

    Article

    Jiang et al Oncogene. 2008 Sep 25. 27(43):5729-40.

    Insert

    Ras-homolog enriched in brain (RHEB Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    glutamine 64 to leucine

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pET3a-Ub-K63R

    Plasmid

    #18898

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin-K63R (UBC Human)
    

    Expression

    Bacterial

    Mutation

    Lysine 63 is replaced with a Argnine residue

    Available Since

    Sept. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEERM3 Km

    Plasmid

    #64025

    Purpose

    Plasmid for integration into neutral site of Synechocystis 6803

    Depositor

    Pia Lindberg

    Article

    Englund et al ACS Synth Biol. 2015 Jul 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PnrsB

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgex-cIAP1

    Plasmid

    #8328

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pBHM2406

    Plasmid

    #173442

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-HYGB
    

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSC234

    Plasmid

    #216357

    Purpose

    xhNup93-Nb3t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup93-Nb3t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115207

    Purpose

    Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    BzoCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRBV5-1

    Plasmid

    #123414

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV5-1 (TRBV5-1 Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPP5052

    Plasmid

    #128729

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAM1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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