30,803 results

1. pGDP3 cat

   Plasmid

   #112888

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   cat (Brevibacillus brevis) (cat Brevibacillus brevis)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDE43-MCSq17

   Plasmid

   #27333

   Depositor

   Dirk Schnappinger

   Article

   Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

   Insert

   qtag17
   

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCBFD2Bad

   Plasmid

   #53574

   Purpose

   Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

   Insert

   cbfd2
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29a-hNABP1

   Plasmid

   #128306

   Purpose

   Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-HA-GAAZFP-FokI-RR

   Plasmid

   #18754

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   zinc finger nuclease
   

   Expression

   Bacterial

   Mutation

   RNA expression vector for expressing the zinc fin…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   see manuscript

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p404TEF1

   Plasmid

   #15972

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   TEF1 promoter is between SacI and XbaI restrictio…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_GNAQ_G-alpha

   Plasmid

   #109809

   Purpose

   Protein expression and purification of GNAQ_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAQ_G-alpha (GNAQ Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AWP-029

   Plasmid

   #213966

   Purpose

   Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

   Depositor

   Alexander Westermann

   Article

   Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

   Insert

   Cas12a (cas12a Synthetic, Segatella bryantii)
   

   Tags

   3XFLAG

   Expression

   Bacterial

   Mutation

   D875A to inactivate target nuclease activity

   Promoter

   PcfxA, IPTG inducible

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-CUL5-pCMV7.1

    Plasmid

    #155024

    Purpose

    N-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 5 (CUL5 Human)
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    pCMV7.1

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD33.lon

    Plasmid

    #22145

    Depositor

    Robert Sauer

    Article

    Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

    Insert

    Lon (lon E. coli K12)
    

    Expression

    Bacterial

    Mutation

    None, WT gene

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDsgRNA-FRT

    Plasmid

    #183241

    Purpose

    anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

    Depositor

    Karin Schnetz

    Article

    Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

    Insert

    sgRNA-FRT
    

    Expression

    Bacterial

    Promoter

    P-tet

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP2844

    Plasmid

    #160675

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mKate1.31-pBAD

    Plasmid

    #54827

    Purpose

    Localization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Type

    Empty backbone

    Tags

    6xHis and mKate1.31 (aka mKate2)

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET-His6MBP-A3A-Gly6His6

    Plasmid

    #187822

    Purpose

    Expression human APOBEC3A in E.coli.

    Depositor

    Hao Wu

    Article

    Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

    Insert

    APOBEC3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter (on the backbone)

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ec dnaN-pHK/P

    Plasmid

    #237239

    Purpose

    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

    Depositor

    Michael O'Donnell

    Insert

    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    

    Tags

    his-10+PKA kinase motif+PreScission Protease clea…

    Expression

    Bacterial

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

    Plasmid

    #235150

    Purpose

    Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    HaloTag linked to SpyCatcher3
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBWB514

    Plasmid

    #209326

    Purpose

    p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

    Depositor

    Adam Arkin

    Article

    Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

    Insert

    mRFP
    

    Expression

    Bacterial

    Promoter

    tet

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEL2319

    Plasmid

    #195697

    Purpose

    Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    Foldon-MlaC
    

    Expression

    Bacterial

    Mutation

    6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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