30,535 results

1. p405ADH1

   Plasmid

   #15966

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p405ADH1 (ADH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   ADH1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-GlyRS-His

   Plasmid

   #124110

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDx_mScarlet3-SYFP2

   Plasmid

   #189764

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET23b-Casp3-His

   Plasmid

   #11821

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM5505

   Plasmid

   #132664

   Purpose

   pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Insert

   MobA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   please see depositor comments below

   Promoter

   p1,p3

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lac-I-SceI-Tet

   Plasmid

   #17655

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   lac I-SceI tet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 30, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pTOX6

   Plasmid

   #127451

   Purpose

   Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   tse2 toxin
   amilCP chromoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   pRha and tac

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVSV eGFP RABV-G

   Plasmid

   #31833

   Depositor

   Connie Cepko

   Article

   Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   VSV genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAJE3-E7

   Plasmid

   #214359

   Purpose

   Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

   Depositor

   Ryan Mehl

   Article

   Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

   Inserts

   2nd generation Tet2 "E7" aminoacyl tRNA synthetase
   Lpp promoted M. jannaschii tRNA
   Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
   Orthogonal "D4II" ColE1 origin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AmpR and lpp

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Use

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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