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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHBintE

Plasmid

#48136

Purpose

Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

Depositor

Dieter Jahn

Article

Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE

Plasmid

#188946

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR AE scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Aqp4-eGFP

Plasmid

#176747

Purpose

Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pExp-Lipo

Plasmid

#129237

Purpose

Protein expression in E.coli

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

His and Strep

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKD236-4b

Plasmid

#90956

Purpose

Expresses ThsS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Bba_J23104

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS mCherry

Plasmid

#91769

Purpose

Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Cherry

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

See Depositor Comments

Promoter

T7

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAM007

Plasmid

#170011

Purpose

Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PslyB

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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