30,535 results

1. pET30a-His6-LOXCAT

   Plasmid

   #140084

   Purpose

   Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBud-EGFP

   Plasmid

   #23027

   Depositor

   Andrea LeBlanc

   Article

   Jodoin et al PLoS One. 2009 . 4(8):e6647.

   Insert

   EGFP (eGFP )
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETa mEos2

   Plasmid

   #20341

   Purpose

   bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

   Depositor

   Loren Looger

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   mEos-2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc396

   Plasmid

   #161954

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-ldhGFP

   Plasmid

   #27170

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBFC1171

   Plasmid

   #231994

   Purpose

   CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Insert

   crRNA of dRfxCas13d
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJEx (Jungle Express)

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

    Plasmid

    #199248

    Purpose

    Fluorescent labelling of bacterial cells with sfGFP

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized sfGFP gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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