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pBTG2

Plasmid

#149475

Purpose

Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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KE-009

Plasmid

#133441

Purpose

Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

Depositor

Gerhard Wagner

Insert

EIF4H (EIF4H Human)

Expression

Bacterial

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Promoter

PtetA

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2plus

Plasmid

#164226

Purpose

Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

Depositor

Silvia Cardona

Article

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Expression

Bacterial

Promoter

Rhamnose-inducible

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAB744

Plasmid

#176579

Purpose

Expressing the whole n-butanol pathway.

Depositor

Arpita Bose

Article

Bai et al Commun Biol. 2021 Nov 3;4(1):1257. doi:

Insert

phaJ, ter, adhE2, phaA, phaB

Expression

Bacterial

Mutation

None

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)3

Plasmid

#127093

Purpose

Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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K560GFP

Plasmid

#129761

Purpose

Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

Depositor

William Hancock

Insert

KHC (Khc Fly)

Tags

GFP and His6

Expression

Bacterial

Mutation

Truncation at 559

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_DYNLL1_Dynein-light

Plasmid

#110006

Purpose

Protein expression and purification of DYNLL1_Dynein-light

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DYNLL1_Dynein-light (DYNLL1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pACYC-LIC+

Plasmid

#62312

Purpose

E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and thrombin protease cleavage site

Expression

Bacterial

Promoter

T7-lacO

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
AsCpf1-2NLS

Plasmid

#102565

Purpose

bacterial expression of AsCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLAM12

Plasmid

#26908

Depositor

Graham Hatfull

Article

van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

Insert

Acetamidase Promoter

Expression

Bacterial

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only
pTYB2-eIF1A

Plasmid

#37218

Depositor

Jon Lorsch

Article

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF1A

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pET28a_MBP_RBFOX2

Plasmid

#224292

Purpose

MBP-RBFOX2 protein purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 (RBFOX2 Human)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110572

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD-stRBS-aacC1

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP_PCNA_IRES_puro2b

Plasmid

#21048

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

hPCNA

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only

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