29,776 results

1. pGEX-2T-GST-eIF4E K119A

   Plasmid

   #112818

   Purpose

   Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Eif4e (Eif4e Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K119 mutated to A, increasing affinity for cap st…

   Promoter

   T7

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLG016 (ApeA)

   Plasmid

   #157894

   Purpose

   Expresses the ApeA defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ApeA (putative HEPN RNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL0M-S-mTurquoise2-EC18152

   Plasmid

   #137074

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

   Plasmid

   #71755

   Purpose

   His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2_(pos36_GFP)-LPETG-His
   

   Use

   Tags

   6XHis, Sortase (LPETG), and TEV Protease

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_mouse-Caspase-11_enzymatic-domain

   Plasmid

   #183382

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-11 (AA100-373) (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-DsbC

   Plasmid

   #112564

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-DsbC and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCBP1_Full-length_pGEX

   Plasmid

   #135108

   Purpose

   Recombinant expression of PCBP1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP1 (PCBP1 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCSG53_N

   Plasmid

   #167255

   Purpose

   Bacterial expression of Nucleocapsid protein (residues 1-419)

   Depositor

   Alexei Savchenko

   Insert

   N protein (residues 1-419) (N SARS-CoV-2)
   

   Use

   Tags

   His6-TEV site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   April 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. TJF229

   Plasmid

   #132575

   Purpose

   pZE1-oxySp-mCherry-proD-oxyR

   Depositor

   Timothy Lu

   Article

   Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

   Inserts

   oxySp-mCherry-TermT1
   proD-oxyR-TermT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   oxySp and proD

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-5X1 Ash2L full

    Plasmid

    #15549

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Francisco Malagon, Sankar Adhya

    Insert

    superfolder GFP
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET19b+H2B(T116C)

    Plasmid

    #157808

    Purpose

    Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H2B(T116C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T116C

    Promoter

    T7

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKSJ331

    Plasmid

    #103063

    Purpose

    Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

    Depositor

    Karen Jakes, Alan Finkelstein

    Article

    Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

    Insert

    Colicin E1 and E1 immunity protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    colicin promotor from ColE1

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPSU100

    Plasmid

    #173835

    Purpose

    Expresses 100 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bQRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T HHARI RBR

    Plasmid

    #31254

    Depositor

    Rachel Klevit

    Article

    Wenzel et al Nature. 2011 May 1. ():.

    Insert

    Human Homologue of Ariadne (HHARI) (ARIH1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    RBR domain residues 177-395

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pPP537

    Plasmid

    #158803

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    2xSV40NLS-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. K980

    Plasmid

    #129762

    Purpose

    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

    Plasmid

    #40019

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLZ12Km2-P23R:TA:ffluc

    Plasmid

    #88900

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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