30,296 results

1. Fast-GCaMP6f-RS09

   Plasmid

   #67160

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Badura et al Neurophotonics. 2014 Oct;1(2):025008.

   Insert

   Fast-GCaMP6f-RS09
   

   Use

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Mutation

   L414T within GCaMP6f

   Promoter

   T7

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJYS2cm_crtYf

   Plasmid

   #85543

   Purpose

   Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-21a(+)/MBP-His

   Plasmid

   #38006

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   MBP (Maltose binding protein)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pBR322-Pdp1-HiBiT

   Plasmid

   #198310

   Purpose

   Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCascade_SPS1(PmcCAST)

   Plasmid

   #224926

   Purpose

   Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

   Depositor

   Leifu Chang

   Article

   Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

   Inserts

   Cas5, Cas6, Cas7, Cas8, Cas11
   CRISPR assay for crRNA expression
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET_StrepII_TEV_LIC_TniQ(PmcCAST)

   Plasmid

   #224927

   Purpose

   TniQ expression plasmid for the co-purification of TnsC-TniQ complex

   Depositor

   Leifu Chang

   Article

   Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

   Insert

   TniQ
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXD80RFP-pXD68Kan2-RFP

   Plasmid

   #225329

   Purpose

   RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   RFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. QUAS-5-T7-TetO-GFP_ColE1

   Plasmid

   #171660

   Purpose

   QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-5-T7-TetO-GFP ssra-T7 Stop
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7-LacO-QF-T7-TetO-TetR_p15A

   Plasmid

   #171661

   Purpose

   Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QF-T7-TetO-TetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-parkin WT

    Plasmid

    #45969

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE-Tn7-135

    Plasmid

    #118553

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-AMVpl-δWT-nAchR

    Plasmid

    #97188

    Purpose

    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTDpelB-C_sfYFPTwinStrep

    Plasmid

    #45944

    Purpose

    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    

    Use

    Tags

    PelB export sequence and Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq/Ptrc

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSETa-mMaple3-V157I

    Plasmid

    #98577

    Purpose

    photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mMaple3-V157I
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TAF3 PHD_pGEX-6P-2

    Plasmid

    #92100

    Purpose

    Expresses GST fusion TAF3 PHD domain protein

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

    Insert

    TAF3 PHD domain (TAF3 Human)
    

    Use

    Tags

    GST Tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_mNeongreen-Etanercept

    Plasmid

    #182420

    Purpose

    Bacterial expression of mNeongreen-Etanercept fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-Etanercept (TNFRSF1B Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PfICH pET15b

    Plasmid

    #60684

    Purpose

    Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

    Insert

    inhA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-PuroDTK.Neo

    Plasmid

    #72307

    Purpose

    PiggyBac Cassette contains puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Insert

    Puromycin-DTK-neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 Set9 wt

    Plasmid

    #24082

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only