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pMRE-Tn5-135

Plasmid

#118521

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_PflaB-mCherryBb

Plasmid

#118243

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE136

Plasmid

#118490

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-133

Plasmid

#118551

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE162

Plasmid

#118510

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE160

Plasmid

#118508

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

Plasmid

#110201

Purpose

Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

LexPR transactivator - 2A - Cerulean

Tags

Cerulean

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-151

Plasmid

#118565

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-156

Plasmid

#118570

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-163

Plasmid

#118575

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDC158

Plasmid

#115793

Purpose

Expresses Rat SNAP25

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

SNAP25 (Snap25 Rat)

Tags

hexa histidine

Expression

Bacterial

Mutation

c84s, c85s, c90s, c92s

Promoter

T7

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDC216

Plasmid

#115791

Purpose

Expresses rat Syntaxin 1

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Syntaxin1 (Stx1a Rat)

Tags

hexa histidine

Expression

Bacterial

Mutation

C145S S193C C271S C272S

Promoter

T7

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG291

Plasmid

#115605

Purpose

surface-displayed Null, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG288

Plasmid

#115607

Purpose

surface-displayed Ag3, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG287

Plasmid

#115606

Purpose

surface-displayed Ag2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

Plasmid

#115218

Purpose

Expresses PguCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PguCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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AFDN_PDZ_1

Plasmid

#103938

Purpose

Protein expression and purification of TRITHORAX PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

AFDN (AFDN Human)

Tags

GST

Expression

Bacterial

Mutation

contains AA983-1110 of AFDN

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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