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34,231 results

Showing: 5201 - 5220 of 34231 results

  1. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Publication
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  2. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Publication
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  6. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  7. pWZ403

    Plasmid
    #208113
    Purpose
    mSwAP-In marker cassette 1 with mScarlet-PuroTK
    Depositor
    Jef Boeke
    Publication
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet-PuroTK
    Tags
    P2A
    Expression
    Bacterial and Mammalian
    Promoter
    EF1alpha
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    208113-plasmid-map-sequence-id-413316

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  10. pJAB988

    Plasmid
    #133920
    Purpose
    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter
    Depositor
    Christopher Voigt
    Publication
    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.
    Insert
    miniICE-tet(M)cat(PC194)_PT7-GFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    133920-plasmid-map-sequence-id-409802

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  17. pCDF-Mm2

    Plasmid
    #197100
    Purpose
    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati
    Depositor
    Kensaku Sakamoto
    Publication
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    PylRS variant, Pyl tRNA, kanamycin resistance gene
    Expression
    Bacterial
    Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197100-plasmid-map-sequence-id-393189

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  18. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Publication
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

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Showing: 5201 - 5220 of 34231 results