30,807 results

1. pET28_human-Caspase-4_enzymatic-domain

   Plasmid

   #183381

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4 (AA94-377) (CASP4 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbS8c-ddcpf1-Δ

   Plasmid

   #153039

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Expression

   Bacterial

   Mutation

   E993A

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSW039

   Plasmid

   #140035

   Purpose

   KO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Yellow fluorescent protein
   

   Expression

   Bacterial

   Promoter

   cLac143

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScI_dCas9-CDA

   Plasmid

   #108549

   Purpose

   Bacterial Target-AID vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1
   

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExp-Skp

   Plasmid

   #129241

   Purpose

   Protein expression in E.coli

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   His and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Galectin-8 FL

   Plasmid

   #68805

   Purpose

   Express Galectin-8 FL protein in Bacterial cells

   Depositor

   Hyun Kyu Song

   Article

   Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

   Insert

   Galectin-8 (LGALS8 Human)
   

   Tags

   N-terminal 6-histidine-tag

   Expression

   Bacterial

   Mutation

   No mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. 6his-WT gusA-pET28a+

   Plasmid

   #61156

   Purpose

   N -terminal His tagged wild-type GUS expression vector

   Depositor

   Andrew Ellington, Ichiro Matsumura

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   gusA
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNLS-2xBFP(4C)

   Plasmid

   #176151

   Purpose

   Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   NLS-2xBFP(4C)
   

   Tags

   6xHis tag and SV40 nuclear localization sequence …

   Expression

   Bacterial

   Mutation

   Compared with EGFP, the two BFP domains have the …

   Promoter

   Ptrc

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. UAS:TVA-mCherry

    Plasmid

    #187823

    Purpose

    Expression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.

    Depositor

    Rainer Friedrich

    Article

    Satou et al Elife. 2022 Jul 22;11. pii: 77153. doi:

    Insert

    TVA-mCherry
    

    Expression

    Bacterial

    Promoter

    5xUAS

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAJM.1642

    Plasmid

    #108535

    Purpose

    CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CinRAM + PCin-YFP
    

    Expression

    Bacterial

    Mutation

    CinR: S72C V80I G114D G115D R137H K220R A224T *24…

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL017

    Plasmid

    #53532

    Purpose

    This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpBA
    

    Tags

    6His and myc

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-mISG15(GG)

    Plasmid

    #12448

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Methods Enzymol. 2005;398:491-9. doi: 10

    Insert

    C-terminal processed form of ISG15
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pGBW-m4046959

    Plasmid

    #145722

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. SNX10-PX (1-201)

    Plasmid

    #119091

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX10-PX (1-201) (SNX10 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pThermeleon

    Plasmid

    #86120

    Purpose

    Bandpass temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

    Promoter

    pR/pL, pR/pL, pLacI, and pTlpA

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDest

    Plasmid

    #124245

    Purpose

    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-32a(+)-TGFB1

    Plasmid

    #120361

    Purpose

    Produce human recombinant TGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. ETS1_pet28a

    Plasmid

    #131663

    Purpose

    Bacterial expression of ETS gene ETS1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETS1 (ETS1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST17-UbE2L6

    Plasmid

    #15797

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2L6 (UBE2L6 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only