Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

33,449 results

Showing: 5241 - 5260 of 33449 results

  1. pET30a-His6-ABP-LOXCATmut

    Plasmid
    #139972
    Purpose
    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Publication
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139972-plasmid-map-sequence-id-311162

    Enlarge

  2. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

    Enlarge

  4. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

    Enlarge

  6. pETM33_Nsp4

    Plasmid
    #156474
    Purpose
    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp4 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156474-plasmid-map-sequence-id-307734

    Enlarge

  9. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

    Enlarge

  10. pGBW-m4092709

    Plasmid
    #153859
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    Nearly native encoding;handful SNPs as watermarks…
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153859-plasmid-map-sequence-id-301594

    Enlarge

  12. pBAD-MBP-His-SFP

    Plasmid
    #141141
    Purpose
    Expression of 4'-phosphopantetheinyl transferase gene
    Depositor
    New England Biolabs
    Insert
    4'-phosphopantetheinyl transferase
    Tags
    MBP
    Expression
    Bacterial
    Available since
    May 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    141141-plasmid-map-sequence-id-298488

    Enlarge

  13. pGEX:PGRMC1

    Plasmid
    #136062
    Purpose
    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.
    Depositor
    Francisco Domínguez Hernández
    Publication
    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066
    Insert
    Progesterone receptor membrane component 1 (PGRMC1 Human)
    Tags
    GST-PGRMC1
    Expression
    Bacterial
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136062-plasmid-map-sequence-id-272028

    Enlarge

  15. pGBW-m4136461

    Plasmid
    #149538
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149538-plasmid-map-sequence-id-307561

    Enlarge

  16. pGBW-m4046910

    Plasmid
    #145672
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145672-plasmid-map-sequence-id-297836

    Enlarge

  17. pGBW-m4046806

    Plasmid
    #145746
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145746-plasmid-map-sequence-id-292514

    Enlarge

  18. pGBW-m4046916

    Plasmid
    #145627
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145627-plasmid-map-sequence-id-292495

    Enlarge

  19. pGBW-m4046833

    Plasmid
    #145649
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp9
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145649-plasmid-map-sequence-id-292493

    Enlarge

  20. pGBW-m4046871

    Plasmid
    #145724
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp10
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145724-plasmid-map-sequence-id-292490

    Enlarge

Showing: 5241 - 5260 of 33449 results